Novel partiti-like viruses are conditional mutualistic symbionts in their normal lepidopteran host, African armyworm, but parasitic in a novel host, Fall armyworm

Autoři: Pengjun Xu aff001;  Liyu Yang aff001;  Xianming Yang aff003;  Tong Li aff004;  Robert I. Graham aff005;  Kongming Wu aff003;  Kenneth Wilson aff002
Působiště autorů: Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao, China aff001;  Lancaster Environment Centre, Lancaster University, Lancaster, United Kingdom aff002;  State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China aff003;  Institute of Plant Protection, Henan Academy of Agricultural Sciences, Zhengzhou, China aff004;  Department of Animal and Agriculture, Hartpury University, Gloucester, United Kingdom aff005
Vyšlo v časopise: Novel partiti-like viruses are conditional mutualistic symbionts in their normal lepidopteran host, African armyworm, but parasitic in a novel host, Fall armyworm. PLoS Pathog 16(6): e32767. doi:10.1371/journal.ppat.1008467
Kategorie: Research Article
doi: 10.1371/journal.ppat.1008467


Recent advances in next generation sequencing (NGS) (e.g. metagenomic and transcriptomic sequencing) have facilitated the discovery of a large number of new insect viruses, but the characterization of these viruses is still in its infancy. Here, we report the discovery, using RNA-seq, of three new partiti-like viruses from African armyworm, Spodoptera exempta (Lepidoptera: Noctuidae), which are all vertically-transmitted transovarially from mother to offspring with high efficiency. Experimental studies show that the viruses reduce their host’s growth rate and reproduction, but enhance their resistance to a nucleopolyhedrovirus (NPV). Via microinjection, these partiti-like viruses were transinfected into a novel host, a newly-invasive crop pest in sub-Saharan Africa (SSA), the Fall armyworm, S. frugiperda. This revealed that in this new host, these viruses appear to be deleterious without any detectable benefit; reducing their new host’s reproductive rate and increasing their susceptibility to NPV. Thus, the partiti-like viruses appear to be conditional mutualistic symbionts in their normal host, S. exempta, but parasitic in the novel host, S. frugiperda. Transcriptome analysis of S. exempta and S. frugiperda infected, or not, with the partiti-like viruses indicates that the viruses may regulate pathways related to immunity and reproduction. These findings suggest a possible pest management strategy via the artificial host-shift of novel viruses discovered by NGS.

Klíčová slova:

Host-pathogen interactions – Larvae – Microinjection – Pupae – RNA viruses – Viral gene expression – Viral transmission and infection – Spodoptera


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