Selective fragmentation of the trans-Golgi apparatus by Rickettsia rickettsii


Autoři: Karin Aistleitner aff001;  Tina Clark aff001;  Cheryl Dooley aff001;  Ted Hackstadt aff001
Působiště autorů: Host-Parasite Interactions Section, Laboratory of Bacteriology, Rocky Mountain Laboratories, NIAID, NIH, Hamilton, Montana, United States of America aff001
Vyšlo v časopise: Selective fragmentation of the trans-Golgi apparatus by Rickettsia rickettsii. PLoS Pathog 16(5): e32767. doi:10.1371/journal.ppat.1008582
Kategorie: Research Article
doi: 10.1371/journal.ppat.1008582

Souhrn

Fragmentation of the Golgi apparatus is observed during a number of physiological processes including mitosis and apoptosis, but also occurs in pathological states such as neurodegenerative diseases and some infectious diseases. Here we show that highly virulent strains of Rickettsia rickettsii, the causative agent of Rocky Mountain spotted fever, induce selective fragmentation of the trans-Golgi network (TGN) soon after infection of host cells by secretion of the effector protein Rickettsial Ankyrin Repeat Protein 2 (RARP2). Remarkably, this fragmentation is pronounced for the trans-Golgi network but the cis-Golgi remains largely intact and appropriately localized. Thus R. rickettsii targets specifically the TGN and not the entire Golgi apparatus. Dispersal of the TGN is mediated by the secreted effector protein RARP2, a recently identified type IV secreted effector that is a member of the clan CD cysteine proteases. Site-directed mutagenesis of a predicted cysteine protease active site in RARP2 prevents TGN disruption. General protein transport to the cell surface is severely impacted in cells infected with virulent strains of R. rickettsii. These findings suggest a novel manipulation of cellular organization by an obligate intracellular bacterium to determine interactions with the host cell.

Klíčová slova:

Golgi apparatus – Host cells – Intracellular pathogens – Membrane proteins – Protein transport – Vero cells – Virulence factors – Rickettsia rickettsii


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