Exosome-mediated apoptosis pathway during WSSV infection in crustacean mud crab
Autoři:
Yi Gong aff001; Tongtong Kong aff001; Xin Ren aff001; Jiao Chen aff001; Shanmeng Lin aff001; Yueling Zhang aff001; Shengkang Li aff001
Působiště autorů:
Guangdong Provincial Key Laboratory of Marine Biology, Shantou University, Shantou, China
aff001; Institute of Marine Sciences, Shantou University, Shantou, China
aff002; Southern Marine Science and Engineering Guangdong Laboratory, Guangzhou, China
aff003; STU-UMT Joint Shellfish Research Laboratory, Shantou University, Shantou, China
aff004
Vyšlo v časopise:
Exosome-mediated apoptosis pathway during WSSV infection in crustacean mud crab. PLoS Pathog 16(5): e32767. doi:10.1371/journal.ppat.1008366
Kategorie:
Research Article
doi:
https://doi.org/10.1371/journal.ppat.1008366
Souhrn
MicroRNAs are regulatory molecules that can be packaged into exosomes to modulate cellular response of recipients. While the role of exosomes during viral infection is beginning to be appreciated, the involvement of exosomal miRNAs in immunoregulation in invertebrates has not been addressed. Here, we observed that exosomes released from WSSV-injected mud crabs could suppress viral replication by inducing apoptosis of hemocytes. Besides, miR-137 and miR-7847 were found to be less packaged in mud crab exosomes during viral infection, with both miR-137 and miR-7847 shown to negatively regulate apoptosis by targeting the apoptosis-inducing factor (AIF). Our data also revealed that AIF translocated to the nucleus to induce DNA fragmentation, and could competitively bind to HSP70 to disintegrate the HSP70-Bax (Bcl-2-associated X protein) complex, thereby activating the mitochondria apoptosis pathway by freeing Bax. The present finding therefore provides a novel mechanism that underlies the crosstalk between exosomal miRNAs and apoptosis pathway in innate immune response in invertebrates.
Klíčová slova:
Apoptosis – Crabs – Exosomes – Hemocytes – Invertebrates – MicroRNAs – Mitochondria – Viral transmission and infection
Zdroje
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