Effect of the zinc phthalocyanine mediated photodynamic therapy on cytoskeletal apparatus of hela cells


Autoři: Hosikova B 1;  Binder S 1;  Lenobel R 2;  Malohlava J 1;  Hosik J 1;  Jiravova J 1;  Malina L 1;  Zapletalova J 1;  Kolarova H 1
Působiště autorů: Department of Medical Biophysics, Institute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry, Palacky University, Olomouc, Czech Republic 1;  Centre of the Region Hana for Biotechnological and Agricultural Research, Palacky University, Olomouc, Czech Republic 2
Vyšlo v časopise: Lékař a technika - Clinician and Technology No. 2, 2019, 49, 41-45
Kategorie: Původní práce

Souhrn

Photodynamic therapy is a very promising and constantly evolving diagnostic and therapeutic method that is used mainly for malignant and non-malignant tumors treatment. This study deals with the utilization of zinc photosensitizer (λmax ~ 660 nm) from the group of phthalocyanines in photodynamic therapy. The aim of this study is to evaluate in vitro effect of the 5 Jcm-2 zinc phthalocyanine photosensitizer-mediated photodynamic therapy in EC50 concentration (30 nM) on cytoskeletal apparatus of the tumor cell line—HeLa (cervical cancer cells). For the measurement, the tandem mass spectrometry, atomic force and fluorescent confocal microscopy techniques were used. The results showed, that compared to the control cells zinc-derivative mediated photodynamic therapy caused in HeLa cells significant change of the cell height and extensive cytoskeletal actin rearrangement although the levels of beta actin, gamma actin and F-actin did not change significantly. This is probably caused by decreased level of the ARPC2 actin-related protein which is responsible for actin polymerization. Its level decreased 24 hours after therapy by 56%. The cytoskeletal apparatus is one of the basic cellular structures that provides cell shape, cell division and the intracellular transport.  After in vitro 5 Jcm-2 zinc derivative-mediated photodynamic therapy, the cervical carcinoma cells showed a significant damage of the cytoskeletal structure followed by changes of cell shape leading to cell death. Considering these results and low effective concentration (EC50 = 30 nM), the therapy used is potentially very promising antitumor treatment.

Klíčová slova:

Reactive oxygen species – photodynamic therapy – phthalocyanines – cytoskeletal apparatus


Zdroje
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