Comparative analysis of the histological architecture of ovarian tissue following slow-freezing cryopreservation

Česká verze

Authors: Veronika Hončová ¹ ; Jan Vodička ¹ ; Zuzana Slobodová ² ; Radovan Pilka ¹
Authors place of work: Porodnicko-gynekologická klinika LF UP a FN Olomouc ¹; Ústav klinické a molekulární patologie, LF UP a FN Olomouc ²
Published in the journal: Ceska Gynekol 2025; 90(5): 349-359
Category: Původní práce
doi: https://doi.org/10.48095/cccg2025349

Summary

Objective: The aim of this study was to validate the methodology of ovarian tissue cryopreservation using the slow freezing technique as a fertility preservation approach and to assess its potential implementation in clinical practice at the Assisted Reproduction Centre of the University Hospital Olomouc. In parallel, the technical procedure of cryopreservation was optimized and standardized. Materials and methods: The study was conducted between April 2022 and December 2024 at the Department of Obstetrics and Gynecology, University Hospital Olomouc, and included six transgender patients aged 19–25 years who underwent laparoscopic hysterectomy with bilateral adnexectomy as part of gender-affirming surgery. Ovarian tissue obtained during the procedure was immediately processed and cryopreserved using the slow freezing method at the Assisted Reproduction Centre of the University Hospital Olomouc. Histopathological evaluation of the ovarian tissue was performed both prior to cryopreservation and after thawing at the Department of Clinical and Molecular Pathology. Results: Morphological assessment of the ovarian tissue after cryopreservation confirmed preservation of structural characteristics of follicles and stromal components, without signs of significant degeneration. Conclusion: The results confirm that the applied slow freezing protocol for ovarian tissue cryopreservation is appropriate and sufficiently gentle for clinical use. This method represents a reliable option for fertility preservation in patients undergoing gonadectomy, with potential applications in subsequent autologous transplantation or in vitro follicle culture.

Keywords:

assisted reproduction – In vitro fertilization – oncofertility – fertility preservation – ovarian tissue cryopreservation – slow-freezing – transgender

Zdroje

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ORCID autorů

V. Hončová 0000-0001-7066-7853

J. Vodička 0000-0003-0446-4454

Z. Slobodová 0000-0001-7755-048X

R. Pilka 0000-0001-8797-1894

Doručeno/Submitted: 26. 7. 2025

Přijato/Accepted: 30. 7. 2025

Mgr. Veronika Hončová

Porodnicko-gynekologická klinika

LF UP a FN Olomouc

Zdravotníků 248/7

779 00 Olomouc