The long noncoding RNA FRILAIR regulates strawberry fruit ripening by functioning as a noncanonical target mimic

English version

Autoři: Yajun Tang ^aff001; Zhipeng Qu ^aff004; Jiajun Lei ^aff005; Reqing He ^aff002; David L. Adelson ^aff004; Youlin Zhu ^aff002; Zhenbiao Yang ^aff003; Dong Wang ^aff002
Působiště autorů: College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou, China ^aff001; Key Laboratory of Molecular Biology and Gene Engineering in Jiangxi Province, College of Life Science, Nanchang University, Nanchang, China ^aff002; FAFU-UCR Joint Center for Horticultural Biology and Metabolomics, Haixia Institute of Science and Technology, Fujian Agriculture and Forestry University, Fuzhou, China ^aff003; Department of Molecular and Biomedical Science, School of Biological Sciences, The University of Adelaide, Adelaide, Australia ^aff004; College of Horticulture, Shenyang Agricultural University, Shenyang, China ^aff005
Vyšlo v časopise: The long noncoding RNA FRILAIR regulates strawberry fruit ripening by functioning as a noncanonical target mimic. PLoS Genet 17(3): e1009461. doi:10.1371/journal.pgen.1009461
Kategorie: Research Article
doi: https://doi.org/10.1371/journal.pgen.1009461

Souhrn

Long noncoding RNAs (lncRNAs) are emerging as important regulators in plant development, but few of them have been functionally characterized in fruit ripening. Here, we have identified 25,613 lncRNAs from strawberry ripening fruits based on RNA-seq data from poly(A)-depleted libraries and rRNA-depleted libraries, most of which exhibited distinct temporal expression patterns. A novel lncRNA, FRILAIR harbours the miR397 binding site that is highly conserved in diverse strawberry species. FRILAIR overexpression promoted fruit maturation in the Falandi strawberry, which was consistent with the finding from knocking down miR397, which can guide the mRNA cleavage of both FRILAIR and LAC11a (encoding a putative laccase-11-like protein). Moreover, LAC11a mRNA levels were increased in both FRILAIR overexpressing and miR397 knockdown fruits, and accelerated fruit maturation was also found in LAC11a overexpressing fruits. Overall, our study demonstrates that FRILAIR can act as a noncanonical target mimic of miR397 to modulate the expression of LAC11a in the strawberry fruit ripening process.

Klíčová slova:

Biosynthesis – Cloning – Fruits – Gene expression – Leaves – Long non-coding RNA – MicroRNAs – RNA sequencing

Zdroje

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