Next-generation sequencing

Česká verze

Authors: Eva Froňková
Authors place of work: Laboratoř molekulární genetiky CLIP, Klinika dětské hematologie a onkologie 2. LF UK a FN Motol, Praha
Published in the journal: Čes.-slov. Patol., 49, 2013, No. 3, p. 129-132
Category: Přehledový článek

Summary

Next generation or massive parallel sequencing (MPS) is a rapidly advancing method in molecular genetics that will bring significant changes in the personalized medicine field. In this review we briefly describe major types of MPS, including whole-genome, -exome, -transcriptome and amplicon sequencing. We also present an overview of the advantages, drawbacks and possible applications of sequencing technologies available in the Czech Republic.

Keywords:
massive parallel sequencing –⁠ next generation sequencing –⁠ whole-genome –⁠ exome –⁠ transcriptome –⁠ amplicon sequencing

Zdroje

1. Meyerson M, Gabriel S, Getz G. Advances in understanding cancer genomes through second-generation sequencing. Nat Rev Genet 2011; 11(10): 685–696.

2. Welch JS, Link DC. Genomics of AML: clinical applications of next-generation sequencing. Hematology Am Soc Hematol Educ Program 2011; 30–35.

3. Teer JK, Mullikin JC. Exome sequencing: the sweet spot before whole genomes. Hum Mol Genet 2010; 19(R2): R145–151.

4. Gartner J, Davis S, Wei X, et al. Comparative exome sequencing of metastatic lesions provides insights into the mutational progression of melanoma. BMC Genomics 2012; 13 : 505.

5. Malone JH, Oliver B: Microarrays, deep sequencing and the true measure of the transcriptome. BMC Biol 2011; 9 : 34.

6. Logan AC, Gao H, Wang C, Sahaf B, et al. High-throughput VDJ sequencing for quantification of minimal residual disease in chronic lymphocytic leukemia and immune reconstitution assessment. Proc Natl Acad Sci USA 2011; 108(52): 21194 –⁠ 21199.

7. Lister R, Pelizzola M, Dowen RH, et al. Human DNA methylomes at base resolution show widespread epigenomic differences. Nature 2009; 462(7271): 315–322.

8. Glenn TC. Field guide to next-generation DNA sequencers. Mol Ecol Resour 2011; 11 : 759–769.

9. Loman NJ, Misra RV, Dallman TJ, et al. Performance comparison of benchtop high-throughput sequencing platforms. Nat Biotechnol 2012; 30(5): 434–439.

10. Liu L, Li Y, Li S, et al. Comparison of next-generation sequencing systems. J Biomed Biotechnol 2012; 251364.

11. Quail MA, Smith M, Coupland P, et al. A tale of three next generation sequencing platforms: comparison of Ion Torrent, Pacific Biosciences and Illumina MiSeq sequencers. BMC Genomics 2012; 13 : 341.