Vliv autoplazmy obohacené o krevní destičky na tloušťku endometria a citlivost receptorů pro estrogen a progesteron

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Autoři: YYuliya V. Zabrodina ^1,2 ; Konstantin Yu. Boyarsky ^3,4 ; Gainyl U. Akhmedyanova ^1,2 ; Zaituna G. Khamidullina ^1,2
Působiště autorů: Astana Medical University, Astana, Kazakhstan ¹; Department of Obstetrics and Gynecology, No. 1 Astana medical university, Astana, Kazakhstan ²; Department of Obstetrics and Gynecology, S. N. Davydov Pediatric Faculty SZGMU I. I. Mechnikov, Saint-Petersburg, Russia ³; Center for Reproduction, Genesis, Saint-Petersburg, Russia ⁴
Vyšlo v časopise: Ceska Gynekol 2024; 89(3): 203-209
Kategorie: Původní práce
doi: https://doi.org/10.48095/cccg2024203

Souhrn

Cíl: Vliv autoplazmy obohacené o krevní destičky na tloušťku endometria a citlivost receptorů na estrogen a progesteron. Materiál a metody: Do této prospektivní studie bylo zařazeno 200 pacientek. Účastnice studie byly rozděleny do dvou skupin. První, kontrolní skupina dostávala hormonální substituční terapii (HRT –⁠ hormone replacement therapy). Druhá, studijní skupina dostávala intrauterinní infuzi autoplazmy obohacené o krevní destičky (PRP –⁠ platelet-rich autoplasma). Devatenáctý den menstruačního cyklu bylo provedeno ultrazvukové vyšetření s cílem zhodnotit tloušťku endometria a dále imunohistochemická analýza k stanovení citlivosti receptorů pro estrogen a progesteron. Výsledky: Během studie jsme zjistili, že po podání autoplazmy obohacené o krevní destičky se zvýšila tloušťka endometria o 0,85 mm; průměrná tloušťka endometria ve skupině na terapii PRP byla 8,25 mm (8,25–8,61 mm) a ve skupině pacientek, které dostávaly pouze HRT, byla 7,40 mm (7,34–7,65 mm). Citlivost receptorů pro estrogen se v experimentální skupině zvýšila o 3,5, přičemž v této skupině dosáhla hodnoty 75,00 (71,43–74,22) a v kontrolní skupině 71,50 (67,05–70,85). Citlivost receptorů pro progesteron se zvýšila o 9,0, hodnota v této skupině byla 95,0 (91,4–93,8) a v kontrolní skupině 86,0 (83,47–86,27). Závěr: Díky působení destičkových faktorů má terapie PRP pozitivní vliv na endometrium, kdy zvyšuje jeho tloušťku a zlepšuje receptivitu. Je tedy možné vyvodit závěr, že tato metoda zřejmě nalezne velké praktické uplatnění při zlepšování výsledků programů asistované reprodukce.

Klíčová slova:

neplodnost – autoplazma obohacená o krevní destičky – nízké endometrium

Zdroje

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ORCID authors

Y. V. Zabrodina 0000-0001-8972-0300

K. Yu. Boyarsky 0000-0002-2000-1567

G. U. Akhmedyanova 0000-0002-5410-7781

Z. G. Khamidullina 0000-0002-0902-5269

Submitted/Doručené: 4. 12. 2023

Accepted/Přijaté: 23. 1. 2024

Yuliya V. Zabrodina, MD

Astana Medical University

Beibitshilik St 49/A

010000 Astana

Kazakhstan