An Evolutionary Framework for Association Testing in Resequencing Studies
Sequencing technologies are becoming cheap enough to apply to large numbers of study participants and promise to provide new insights into human phenotypes by bringing to light rare and previously unknown genetic variants. We develop a new framework for the analysis of sequence data that incorporates all of the major features of previously proposed approaches, including those focused on allele counts and allele burden, but is both more general and more powerful. We harness population genetic theory to provide prior information on effect sizes and to create a pooling strategy for information from rare variants. Our method, EMMPAT (Evolutionary Mixed Model for Pooled Association Testing), generates a single test per gene (substantially reducing multiple testing concerns), facilitates graphical summaries, and improves the interpretation of results by allowing calculation of attributable variance. Simulations show that, relative to previously used approaches, our method increases the power to detect genes that affect phenotype when natural selection has kept alleles with large effect sizes rare. We demonstrate our approach on a populationbased resequencing study of association between serum triglycerides and variation in ANGPTL4.
Published in the journal:
. PLoS Genet 6(11): e32767. doi:10.1371/journal.pgen.1001202
Category:
Research Article
doi: 10.1371/journal.pgen.1001202
Summary
Sequencing technologies are becoming cheap enough to apply to large numbers of study participants and promise to provide new insights into human phenotypes by bringing to light rare and previously unknown genetic variants. We develop a new framework for the analysis of sequence data that incorporates all of the major features of previously proposed approaches, including those focused on allele counts and allele burden, but is both more general and more powerful. We harness population genetic theory to provide prior information on effect sizes and to create a pooling strategy for information from rare variants. Our method, EMMPAT (Evolutionary Mixed Model for Pooled Association Testing), generates a single test per gene (substantially reducing multiple testing concerns), facilitates graphical summaries, and improves the interpretation of results by allowing calculation of attributable variance. Simulations show that, relative to previously used approaches, our method increases the power to detect genes that affect phenotype when natural selection has kept alleles with large effect sizes rare. We demonstrate our approach on a populationbased resequencing study of association between serum triglycerides and variation in ANGPTL4.
Introduction
Over the past 20 years, positional cloning guided by linkage analysis and genome wide association studies (GWAS) have identified many loci relevant to human disease and other quantitative phenotypes such as height, body mass index, and serum lipid composition. However, in most cases the total amount of phenotypic variance explained is small compared to the heritability observed in twin or adoption studies [1]. Some authors note the possibility that lowfrequency genetic variation, which is not measured on standard single nucleotide polymorphism (SNP) arrays, may contribute to this missing heritability [2]–[7]. The rapidly decreasing cost of obtaining DNA sequence has prompted several groups to test this hypothesis by sequencing candidate genes in participants of cohort or casecontrol studies hoping to discover either 1) rare or previously unknown SNPs with large detectable effect sizes, or 2) a correlation between overall number of rare SNPs and phenotype [8]–[15]. This research is rapidly approaching a new phase as investigators use nextgeneration sequencing technology to measure all variation in the exome and wider genome [16], [17]. Several authors have shown that rare variation is particularly relevant in the case that natural selection has acted to keep variants with large effects rare, and that without action by purifying selection rare variants have effect sizes comparable to common ones [2], [3], [6].
There are three signatures of association in a resequencing study which we want to use to assess candidate genes. Some SNPs could have effect sizes large enough that they have individually noticeable impact on phenotype; this is the information underlying regression procedures, like those put forward by Hoggart et al [18] and Kwee et al [19]. This approach is very similar to current tagSNP based procedures and not designed thinking of resequencing data, since the effects of rare SNPs will not be easy to discern. Depending on the role natural selection has played in the history of the phenotype, two other signatures of association may exist. Second, rare SNPs may tend to have effect sizes in the same direction (e.g. inducing risk), so a measure of overall rarevariant burden could correlate to phenotype; this is the information exploited in allelecount [20] and rarevariantburden [21] type methods. That signature may be present if either selection has favored the phenotype (or a correlate) in a particular direction, or if purifying selection has been weak and derived alleles tend to be deleterious to the phenotype. Finally, rare SNPs could tend to have effect sizes which are larger than common ones. This could be the case if selection has tended to stabilize the phenotype. The method of Kwee et al [19] can allow for that possibility, but does not contain guidance on what the structure of the frequency  effect size relationship should be.
We present a method capable of detecting all three signatures of association. Our method generalizes allele count and rarevariantburden methods by explicitly constructing a model relating disease impact, selective pressure, and SNP frequency in a candidate gene. By doing so, we will be able to provide intuitive interpretations to detected associations, allowing investigators to answer additional questions with their data. Our approach will yield substantially more power if the model is close to correct without introducing bias or sacrificing much efficiency when our assumptions are not met.
We propose to estimate the evolutionary fitness burden of each SNP using its observed frequency and population genetic parameters inferred by other authors. That estimate of fitness burden will act as prior information on the variant effect, acting like a burden function [21]. The same estimate will structure the variability of SNPphenotype correlations, replacing arbitrary weights [19], and provide robust estimates even if there is no relationship between fitness and effect magnitude. We recognize that for a quantitative trait measured in a prospective cohort, a welljustified approximation of the full model can be fit using a fast and general statistical technique, mixed linear models, and provide software routines to estimate parameters and conduct hypothesis tests. We have named the approach EMMPAT (Evolutionary Mixed Model for Pooled Assocation Testing)
In what follows, we will briefly introduce the population genetics ideas which underly our approach. Next, we construct our statistical model and discuss estimation and testing within it. Finally, we illustrate the method both in simulation studies and on a real candidate gene resequencing study examining serum triglyceride levels in a multiethnic prospective communitybased sample [8], [12].
Relating SNP Frequency, Fitness, and Disease Effect
Several authors have reviewed the potential contribution of low frequency alleles to variation in phenotypes [2]–[7]. Absent a change in the properties of new mutations during recent history, which we find implausible, systematic differences between SNPs of varying frequencies must be mediated by natural selection. Since the early 20th century, much work has explicated the evolutionary dynamics of quantitative traits, reviewed by Barton and Johnson [22], [23]. Below we will posit a model of pleiotropic selection whereby the trait under study or a trait with a correlated genetic basis is under purifying selection. More detailed connection and contrast to the existing work on the genetic basis of quantitative traits is found in Text S1.
In Figure 1, we illustrate direct and apparent selection scenarios which give rise to a correlation between fitness effects and phenotype effects. In Figure 1A, the phenotype itself is under selective pressure; for example, disease leading to propensity to childhood mortality. Figure 1B shows apparent selection by pleiotropy; variants which disrupt an unconstrained role of a gene also tend to disrupt another role which is under selection; for example, variation which increases Alzheimer's Disease risk after reproductive age may relate to other brain function which is relevant for individuals still reproducing.
Hartl and Clark [24] carefully constructs and interprets the concept of fitnesseffects in classical population genetics. Briefly, in an idealized population, the relative reproductive advantage of an individual is the product of the fitness effects of each variant that person carries, an additive approximation with no dominance or epistasis. We parameterize the problem in terms of the log of multiplicative fitness effects. That is, the fitness of the person is given by where the fitness effect of the variant is denoted and is the unphased genotype at that locus. The fitness effect of a new mutation determines several of its properties, such as average sojourn time before either going extinct or fixing at 100% prevalence and average frequency when sampled at a point in time [24].
Rather than assume that all variants in the region have the same , we assume that the of new mutations are sampled from a distribution of fitness effects (DFE). Just as a fixed would determine properties of the sampled genotype data for a SNP, a DFE along with mutation, recombination, and demographic parameters induces a distribution on the observed frequency spectrum and polymorphism  divergence ratios in sampled data. Several authors have attempted to fit a parameterized DFE from genomic data [25]–[34]. Boyko et al [33] found that a combination of a point mass at neutrality (not under selection) combined with a gamma distribution for deleterious differences from neutrality to be a good fit for the DFE of nonsynonymous mutations.
With these facts in mind, in what follows we will use fitness effects to operationalize the construct of functional status for each SNP. Whereas Johnson and Barton [23] worked directly with the joint distribution of fitness and phenotype effects, we will use an existing DFE estimate [33] as a marginal distribution for fitness effects and construct the conditional distribution of phenotype effects. Since we do not know the true fitness effects of SNPs, we will estimate them with observed SNP frequency, which is statistically ancillary to phenotypeSNP correlation, using a simulation methodology described below.
Methods
Model for SNP Effects on Phenotype
Assume the context of a simple random crosssectional sample of individuals (indexed by ) studying a quantitative trait measured once per individual. Assume that these individuals also possess vectors of covariates and genotypes at each locus inside a sequenced candidate gene or region. The genotypes are coded such that “0” represents homozygous possession of the ancestral allele, “1” heterozygosity, and “2” homozygous possession of the derived allele at the locus. That is, represents the fourth sampled person possessing two derived alleles at the third locus in the sequenced region.
We can write a regression model for person 's phenotype in terms of deviation from an average level predicted by covariate effects and additive genotype effects ,(1)
Using standard leastsquares regression to estimate such a model will pose several problems. First, because there will be many rare variants, will contain many poorly estimated coefficients. The large number of rare variants will give model (1) a large number of degrees of freedom, decreasing its power to detect association with the candidate gene. Some of the variation uncovered may be perfectly correlated in the sample, meaning that those coefficients are not separately estimable in leastsquares regression. Additionally, as the amount of the genome sequenced becomes large, there will be more variants than participants, making the entire model unidentified.
To overcome these problems, we need to make more assumptions and model the coefficients. We adopt a model where we view the effects of SNPs in the study as a sample from a wider population of SNP effects, and characterize that entire population using only three parameters. To fix ideas, assume for now that we knew the fitness effect of each SNP . If fitness was perfectly correlated to effect on phenotype, we would use that as a summary for all alleles, , where the parameter relates the scales of the two measures. As the fitness effect is not perfectly correlated to effect on phenotype, we add a mean and an error term acknowledging those limitations to obtain(2)In applied problems, is not known apriori, so we will construct a prediction based on the observed frequency. We denote for that estimate, and for its prediction error we write . We plug those estimates in to (2) to obtain(3)and combine the two uncorrelated error terms to yield(4)where(5)(6)The first term in (4), , allows derived alleles to on average increase or decrease the phenotype. The second term is an unscaled correlation between phenotype effects and expected fitness effects . The error term is the deviation in SNP j's effect on phenotype from the average of SNPs with the same observed frequency. The variance of in (6) therefore has two components, first corresponds to prediction error of , and second is the variance of phenotype effects for SNPs at the same level of true fitness burden . The function allows that as average burden changes the variability might also change. Although one could imagine “bad” alleles being more variable in their effects than relatively neutral alleles, implying nonconstant , we propose constant as a reasonable modeling start. This will still allow for the variance of effect sizes to change with observed frequency because of nonuniformity of with frequency.
Equation (4) asserts that phenotypeeffect and fitnesseffect are linearly related; that seems correct for the scenario in Figure 1A and a good starting place for the other possibilities. In future work we will be able to empirically examine this assumption by graphical diagnostics and comparing fits using other functional forms. Further discussion of nonlinear relationships is found in Text S1, and we will demonstrate the impact of an incorrect assumption of linearity in our simulation studies.
Our model is quite general in that existing methods correspond to submodels of (4). An allele count method tests the model with only allowed to vary; rare alleles below an arbitrary threshold are summarized by an average effect which does not change with frequency, so and all are set to zero, and alleles above that threshold are regarded as free parameters. Similarly a weightedburden method corresponds to the model with a particular implementation of , such as in Madsen et al [21] where , and forces all in the rare alleles to be zero. Our model will not involve an arbitrary threshold for “rare alleles” and will adaptively pool variant effects in a flexible way. As shown in the results, this will create substantial power gains in a variety of settings.
When and in (4) are zero, our model reduces to a standard randomeffect model identical to that of Kwee et al [19] with all variants given the same weight. That is, regardless of frequency all SNPs have the same likelihood of having large effect sizes, and regardless of frequency SNP effects have zero mean. As a result, our method will be robust to the case that fitness and phenotype effects are unrelated by estimating and retaining the flexibility of the method of Kwee et al. The major difference between the above and our method is the use of population genetics to suggest the structure of the variance of SNP effects, including a fallback should fitness and phenotype effect not be related. Kwee's method is developed in the context of tag SNPs and suggests an arbitrary variance of SNP effects given as either a constant, , or any priorinformation based form. A related method is that of Hoggart et al [18]. Their approach corresponds to and set to zero (they assume a meanzero distribution) and a different set of restrictions on the distribution of . Their assumptions about the distribution of were chosen to yield estimates with most variants having zero effect, a feature called model selection which eliminates small effects and correlated variables. In contrast, our model will tend to reign in large effect sizes and split effect size between variants in high linkage disequilibrium, but does not eliminate SNPs from the fit. We prefer our choice for resequencing for several reasons. First, there may well be many effects of small size which are cumulatively important, and we want to retain those small effects in the model. Second, we want an estimate of the effect size of each variant for graphical and diagnostic purposes. Third, we accomplish a similar goal of reducing the model size by rejecting the null on a small number of genes. That is, we want to identify a small number of disease relevant genes with our efficient test; doing so will exclude most SNPs without further model selection procedures. Fourth, by smoothly grouping rare SNPs and summarizing them with only a few parameters, we already greatly reduce the multiple testing burden.
Model Interpretation
The specification of equations (1), (4), and (6) yields a natural interpretation to the fitted model. After estimating the population parameters of phenotype effects, we will be able to jointly estimate individual SNP effects and their impact on the phenotype of each person in our sample. By calculating , we obtain the expected difference between participant i's phenotype and what we would expect were there no effects of this gene. As a result we can empirically estimate the overall phenotypic variability due to observed genetic variants, over study participants. We can similarly estimate the variability dues to rare alleles by including only rare SNPs in the above calculation. The overall effect is an average change in phenotype per derived allele, perhaps due to inadequate purifying selection. In the variance expression (6), is the variability of allelic effects for a given level of true fitness. As will be shown below in Figure 2, when using the genomewide distribution of fitness effects for nonsynonymous SNPs, common variation is nearly neutral so can also be thought of as the variability of effects of common alleles. represents the correlation between fitness burden and phenotypic burden. This parameter's interpretation relies on accurately estimating the scale of fitness effects and has awkward units, but we can avoid this difficulty by noting that (4) can be decomposed into a fitness related portion and a fitness unrelated portion which are independent(7)By calculating we can ascribe a proportion of total variation in phenotype to selectionphenotype correlation without worrying about having gotten the scale of correct. Calculations for separating these variance components are found in Text S1. We can use the same technique to compare classes of SNPs, for example noncoding vs missense, by jointly fitting separate and comparing the attributable variance for each class of SNPs. We will illustrate this idea in our real data example. This decomposition also shows why it is not crucial for our estimates of fitness to be perfect. The model can fall back by setting to zero and use only to recover a working model which does not pool information across rare alleles. Doing so will mean that the opportunity to gain information by recognizing structure in phenotype effects will not be realized, but the remaining estimation method is still valid.
An important consideration is how to interpret the results when multiple ethnic groups are analyzed simultaneously. Because some genetic variation is fixed between ethnic groups in the sample, the average effect of singlepopulation variation will be absorbed into the fitted mean for that group. As a result, the interpretation for “total explained variation” is actually “total explained withinethnicgroup variation;” genetic variation may explain some of the phenotypic difference between groups, but we do not include it in our estimate because of confounding between environmental exposures and ethnic background.
Another point requiring clarification is the assumption that genotype effects are independent. In the context of GWAS, nearby SNPs often are thought to have correlated effects because they mutually tag a functional variant. Additionally, estimates of SNP effects will be correlated due to LD making their true separate effects difficult or impossible to identify. However, in the underlying data generating mechanism true genotype effects are independent. Because sequencing identifies all the variation within the region and eliminates much of the correlation due to untyped alleles, we believe that the independence assumption is a useful approximation in this case. Nonindependence of the true effects could be accommodated by imposing a covariance structure on SNP effects, for example using their spatial distance in the genome or folded protein. Alternatively, the phylogenetic approach of TreeLD [35] estimates the degree of probable overlap of untyped SNPs.
Computing Fitness Effects
Model (4) relies on a prediction of the fitness effect of each variant as well as an estimate of the error of that prediction. We use the following procedure to calculate such estimates.

Take as given the fitted distributional form of fitness effects and population history since outofAfrica [33], [36].

Use existing software SFS_CODE [36] to simulate new polymorphisms in the gene under study many times, creating pseudosamples containing true variantlevel fitness.

For each variant in the real dataset, find variants in the pseudodata with the same sampled frequency, and calculate the mean and variance of true fitness among those simulated variants.
To reduce computational requirements, steps 2 and 3 above can be replaced by simulating a smaller number of large populations and calculating the expected mean and variance of fitness using simple random sampling. Figure 2 depicts the relationship of and to frequency when using a genomewide fitted DFE [33]. Because much of the variation discovered in our multiethnic example dataset is confined to one ethnicity, we use the ethnicityspecific frequency and pseudodata. Because of admixture in our sample, we use the highest observed frequency (the most skeptical about its being rare) to assign an ethnicity of origin to SNPs appearing in multiple groups.
An advantage of this method is that because it refers to a feature of genetic history rather than a phenotype, it need only be done once for any trait under study on the same cohort. While the fitness  phenotype relationship will be different for all traits, that is modeled by the fitted parameter rather than modification of . If the impact of LD structure on the prediction does not vary too much between genes, the calculation can be recycled for multiple genes under study. In some experiments, we found the impact of LD to be minimal (data not shown). Discussion of taking the DFE as known versus estimating or using some other flexible function of frequency it is included in Text S1. Discussion of the quality of the existing DFE estimates are also included in Text S1. We have used the observed frequency to estimate the fitness effect, but there are many other potential predictors of functional status. Discussion of including them in our model is found in Text S1.
Model Fitting and Estimation
Testing
Our model fitting procedure will be likelihoodbased, so we will use a standard hypothesis testing method: likelihood ratio tests. To improve robustness, our examples will use permutation pvalues obtained by comparing the likelihood ratio of the fitted model to that generated under the null hypothesis by randomly swapping genotype vectors between members of the same ethnicity. Permuting genotype labels simulates the null hypothesis that no relationship exists between any genotype and any aspect of the response, which in our parametric setup is equivalent to while retaining the relationship between covariates (such as age and sex) and phenotype. Because the genotypes of members of different ethnicities are not exchangeable even under the null, we only swap genotype vectors among individuals with the same reported ethnicity. In admixed populations where information about local ancestry is available, the permutation should be between individuals with the same local ancestry.
Estimation
For numerical convenience and statistical robustness, we will use only the first two moments of the model in equations (1), (4), and (6), and assume constant in (6). This last restriction yields a mixedeffects regression problem where the genotype effects are crossed random factors, presented in (8) and (9) below. A broad introduction to mixed effects regression and many of the formulas we will use are provided in McCulloch and Searle [37]. In matrix notation where each participant is a row and effects are column vectors,(8)(9)
We allow the procedure to exploit the possibility that individuals with a high burden of rare alleles not only have drift in their mean phenotype because of in (8), but also more variability in phenotype due to in (9). Equations (8) and (9) assert that a single parameter regulates the change in mean variant effect and effect variability with frequency. However, nondifferential error (with respect to phenotype) in imputing covariates biases coefficient estimates towards the null, so if our estimations of and have different levels of error they will experience different such biases. As a result, we will want to fit in (8) and in (9) separately to check that they are similar before combining them. Because it involves an extra parameter the “split ” calculation will be more variable under the null and less powerful when the model is true. However, it may be more robust when the model is misspecified, as we will explore in our simulations.
We will fit the mixed effects model (8)–(9) using modified NewtonRaphson optimization of the implied likelihood. The linear mixed effects approach is equivalent to assuming normality for the error terms and and fitting via maximum likelihood. A major advantage of this estimation approach is that it allows for very fast computation; the likelihood can be integrated analytically over when maximizing over parameters and . We have not optimized our software for speed, but it completes in a few seconds for the large example dataset. Though higherorder expansions are possible, others have shown that most of the information is often contained in the first two moments of the data [38], [39], and that correct specification of mean and variance models produces correct inference robust to additional details of structure. Assumptions which better match the data at hand will lead to more power, but they will tend to require dramatically more computational effort. For our current example we have considered a single sequenced candidate gene where computational speed is not crucial, but we expect that methods similar to ours will be required for wholegenome or wholeexome resequencing efforts where computational resources will be a limiting factor. Additionally, popular methods such as Markov Chain Monte Carlo and EM which can use arbitrary distributions of residuals and random effects require accurate initial estimates to perform well; MCMC also benefits enormously from a good proposal distribution. Mixed effect regression is a reasonable way to generate these initializations. Whereas using only the first two moments for estimation is only optimal under the normality of , it will still yield consistent estimates if normality does not hold, and we can use use robust methods of testing the null hypothesis such as permutation pvalues. This quasilikelihoodbased method also yields best linear unbiased estimates for the SNP phenotype effects [37, chapter 6], which we relied upon in “model interpretation”.
Implementation
As discussed above, we will be interested in fitting distinct in (8) and in (9) because of concerns about different magnitudes of error in the computation of and . In such a scenario, we can use the SAS mixed procedure [40] to estimate the model parameters and check our custom software. Example code implementing this use is maintained at the authors' website. We generate confidence intervals using the standard asymptotic arguments in McCulloch and Searle [37, chapter 6], which are built into SAS.
Alternatively, if we use a single in the mean and variance models, the result is a model which is not easily fit in any standard statistics package of which the authors are aware. We have created a set of functions in the R programing language [41] to estimate this model using optim to maximize the likelihood, code for which is posted at the authors' web site: http://home.uchicago.edu/~crk8e/papersup.html
Bayesian interpretation
Our model is easily recast in a purely Bayesian framework. One would need to write priors for and the effects of covariates. The frequentist formulation is just the Bayesian formulation with an improper uniform prior distribution on the variance components. As a result, using Bayesian regression software like R's MCMCglmm package or winBUGs is an alternative for estimation. A reasonable way to generate proper informative priors would be a three step calculation. First, estimate a posterior distribution on variance explained by genetic factors from previous linkage studies. Because many phenotypes may not have available linkage studies or very low resolution, one may have to rely on other phenotypes or animal model results. Second, equate the resulting prior on attributable variance to the expression in Text S1 with observed values for the genotype data. Third, assign an arbitrary fraction of the explained variation to each source and backcalculate to find the square of the parameter.
The Bayesian analyst could continue to use our normal approximation of the distribution of the latent which allows it to be integrated out, or could model it directly including the point mass at zero and skew distribution from the simulation result. The result would be a large model with many latent variables, some of which are poorly identified.
Results/Discussion
Dallas Heart Study: ANGPTL4
Description of dataset
About 3500 prospectively sampled individuals from the population in Dallas, Texas, were sequenced at a candidate gene for dyslipidemia: ANGPTL4 (Ensembl Acc:16039). These individuals come primarily from three ethnic backgrounds: nonHispanic white (N = 1043), nonHispanic black (N = 1832), and Hispanic (N = 601). We will exclude from our analysis the 75 individuals listed as “Other” ethnicity. Our outcome phenotype is logtransformed serum triglyceride levels. Details of the cohort [42], its metabolic phenotypes [43], and the sequencing methods and discovered genetic variation [8], [12] have been described previously. We grouped all missense and nonsense mutations into a single category which we label “nonsynonymous” in the tables and figures, and we grouped all synonymous and noncoding region mutations into a single category labeled “noncoding.” Table 1 shows the number of discovered SNPs in each category in each ethnic group. We consider age, sex, ethnicity, diabetes status, and selfreported ethanol consumption as adjuster covariates. For age, we use a flexible linear spline model with knots at every ten years to allow for nonlinearity in response. We include all interactions between ethnicity and gender and ethnicitygender interactions with other covariates. Because statin use is an endogenous variable indicating diagnosed dyslipidemia, we do not adjust for it. We fit models 1) ignoring statin use and 2) increasing triglyceride levels 25% in the treated to approximate their untreated level. Because we obtained qualitatively similar results, we present only the latter.
Model estimates
Table 2 presents model summaries and point estimates with asymptotic standard errors for model parameters, stratified by ethnicity and pooled using ethnicity as an adjuster. Table 2 presents the results setting the offset term to zero. We found that including in (4) produced poor fits when there were few variants, for example when using only the Hispanic nonsynonymous variants (n = 8). In the pooled estimate, including the offset did not qualitatively change the result.
For ANGPTL4, we observe a pvalue of .006 on 10,000 permutations versus the strong null hypothesis that no SNPs have any effect. Previous authors [12] observed a pvalue for a net surplus of nonsynonymous variants in low triglyceride participants of .016 and a minimum variantatatime pvalue of .019 for E40K corrected for multiple testing. The improvement to the model fit by including is small in this case; a likelihoodratio pvalue using the asymptotic distribution is nonsignificant. As seen in Table 2, a glimmer of a fitness component is only seen in the noncoding variation, and the explained variance is very small. However, to illustrate the interpretation of the plots which our approach generates we'll take the parameter estimates at face value below.
Interpretation of diagnostic plot
Figure 3 shows the observed SNPs and estimated effect sizes (nonsynonymous in black and noncoding in red) rank ordered by observed frequency (in blue). Variantatatime ordinary least squares (OLS) estimates of effect size are overlaid in green. Figure 3 displays several interesting features of the data; first there are two lowfrequency nonsynonymous variants with a strong effect reducing triglyceride levels; the first is E40K (frequency in nonHispanic whites = .012, frequency in nonHispanic blacks = .003), the sole variant identified by Romeo et al [12]. However, adjusted for E40K we see that another more common variant R278Q almost exclusive to nonHispanic blacks (frequency = .055) also appears to decrease triglyceride levels. We observe a weak tendency for all nonsynonymous variation to reduce the phenotype; Romeo [12] also noted an excess of rare nonsynonymous variants in those with low triglyceride levels. The rare noncoding variation appears to have the opposite sign of effect; it increases triglyceride levels. Referring to Table 2 we see that a fitnessrelated component of variability (of about the same scale as the change in mean) was detected; this gives rise to the wider spread of point estimates and wider confidence intervals in noncoding variation.
An interesting data point in Figure 3 is a single 5% frequency noncoding variant (directly before R278Q) whose OLS effect estimate is quite large (and nominally significant) but whose modelbased effect estimate is small. Examining that variant more closely, we found that it is in strong LD with R278Q. Because E40K (which is not strongly correlated to any other variation) had a large effect and nonsynonymous variants tended to decrease triglycerides, the model assigned nonsynonymous variation as more likely to have nonrare variation with large negative effect sizes and gives the effect to R278Q. Similarly, perfectly correlated rare variants have their combined effect split evenly.
We can understand this model fit by looking at the green OLS estimates in Figure 3. Visually, the estimates for nonsynonymous variation tend to be below zero. Comparing the nonsynonymous to noncoding singletons, we see more variable estimates in the noncoding singletons as well as a different mean. The model fit identifies this as opposite signs of and a much greater in noncoding. The nonrare nonsynonymous variants with large effects (E40K, R278Q) drive the larger estimate of versus noncoding variants; examining Figure 2 we see that common variation is essentially neutral with respect to fitness, and as a result nonzero effects in nonrare variants force away from zero.
Evolutionary interpretation
An interesting potential story about natural selection on ANGPTL4 activity emerges from Figure 3. First, nonsynonymous mutation tended to decrease the effectiveness of ANGPTL4 and decrease serum triglyceride levels [8], [44], [45]. We see no evidence of selection against those mutations; variants which decreased triglycerides became more than rare in both the African and European lineages, and we see no excess of large effects in rare SNPs. On the other hand, noncoding mutations which may alter the regulation of ANGPTL4 on average increased triglycerides. Variants with large effect sizes were preferentially rare, and the apparent selective force was stronger in the nonEuropean lineage, as the demographic history would predict. This meshes well with the finding that ANGPTL4 experienced a Europe specific relaxation of purifying selection [12]. We do not suggest that serum triglyceride levels in themselves were the target of purifying selection; effect on triglycerides may only be correlated to effect on a selected function.
Simulation Studies
Population parameters
In order to determine the power and robustness of our procedure, we simulated variation in a gene with the exon structure of the gene ANGPTL4 in a study population using SFS_CODE [36] and fitted demographic and DFE parameters [31], [33]. We used 4cM/mb for the local recombination rate and no recombination hotspots. We used as the mutation rate pernucleotidepergeneration. From the final simulated population of about 20,000 individuals we sampled 1000 individuals independently for each of 1000 simulation runs. SFS_CODE commands creating the simulated population are available at the authors' web site. We created simulated phenotypes according to (1) and (2) using parameters described below. The total simulated population had 132 codingregion SNPs, 29 of which were at frequency greater than 1%.
Model parameters
We chose several levels of the phenotype parameters to correspond to potential cases of interest while keeping the total fraction of variation explained by the gene about the same: a weak mean variant effect, a strong fitnessrelated component of the phenotype, and a strong fitness independent component of the phenotype. We chose the baseline values such that and explain about the same amount of variation in phenotype. We also created a scenario with no fitnessphenotype correlation whatsoever. To ensure that type 1 error rates were correct, we include a simulation under the null hypothesis that no variants have any effect on phenotype. Table 3 contains the chosen phenotype parameter values for each set of simulations and the resulting expected percent of variance explained by the SNPs due to fitnessphenotype correlation and percent of variance explained independent of that correlation.
Two additional batches of simulation examine the robustness of our procedure to incorrect assumptions. First we created violations of the assumed population model. We misspecified the assumed DFE in our analysis, making the scale parameter a factor of 5 too large or too small and keeping the truth the same. We also simulated violation of our demographic assumptions using a population which experienced an additional 100 fold exponential growth over the last 11% of generations since outofAfrica. Second we created violations of the assumed statistical model. We simulated three scenarios violating the linearity assumption. First, with proportional to , second proportional to , and third a 50/50 mixture of and . We simulated using a highly skewed lognormal distribution which was then standardized to have mean zero and variance . We also simulated with 20% and 80% of the variants having an effect size of zero.
Power comparisons
To compare power with existing methods, we included several proposed methods of analysis. First, we test the method of Bonferroni corrected minimum pvalue of SNPs with minor allele frequency >1% or >5%. Other proposed methods using allele counts like CAST [46] CMC [20] and weighted sums [21] were created for casecontrol studies, so we alter those methods to be fair in a cohort quantitativetrait context. Our representative of CASTlike analysis is regression with the number of rare variants carried by each participant as a covariate; CMClike analysis is the same with nonrare SNPs (frequency greater then 1% or 5%) treated as free regression parameters. Pvalues are then generated by ANOVA against the nested model consisting only of only fitting the mean response. Our representative of weightedsum type methods is a similar regression analysis where rare variants are collapsed to a mean model with burden proportional to , which is the same weight used by Madsen et al [21]. Because the simulated response is actually normal, we do not use a rank transformation. We also used the same burden function for only low frequency SNPs and treated common SNPs as regression parameters. Pvalues are again obtained by ANOVA versus a nested model with no genetic effects.
To demonstrate the gain (or loss) in information by considering the marginal variance, we apply a similar regression with an optimal mean model, that is (8) either for all SNPs or treating common SNPs as free. We tested our model both with a single in the mean and variance and the “split ” calculation where separate parameters are fit in (8) and (9).
Table 4 summarizes the power comparisons in each case. Our model is as or more powerful than the existing methods, even when there is substantial violation of its assumptions. The only scenario in which our model loses some power is when there is absolutely no fitnessphenotype correlation. Even in that case, the relative loss is small, much smaller than the gain when was not zero. The additional utility of the method varies substantially depending on the chosen parameters. For example, when the fitnessphenotype correlation accounts for about half the genetic component of the phenotype (the basic scenario), our method provides a substantial improvement, but when is large (common variants have large effect sizes) the benefit is less. Our model appears reasonably robust to all the violations of assumptions which we tested, even providing a performance benefit when effect sizes were very skew or the true relationship was nonlinear. In effect, the truth in those cases lined up less well with the implicit assumptions of the competing methods. Perhaps most importantly, even fairly substantial mistakes in the DFE and demographic history did not dramatically reduce the power of our method. The “split ” model appears to perform about the same as a single . The minimum pvalue method's poor showing in some scenarios is explained by the data generating mechanism we chose; when is small or many SNPs have zero effect there will often be no common variants with appreciable effect sizes.
Discussion
We propose a novel method, EMMPAT, for association between sequenced genes and phenotype which utilizes population genetic theory to pool information among rare variants. Our method generalizes allelecount and alleleburden techniques, and presents several advantages. Of greatest importance to the practicing scientist will be increased power and interpretability. As shown above, our method allows us to leverage allele frequency as auxiliary data related to SNP effects and to substantially increase power to detect association in many scenarios. The availability of a well motivated pooling strategy allows an omnibus test which incorporates common and rare variation simultaneously. Our approach provides clear interpretations for the fitted model, such as the attributable variance in phenotype due to all polymorphisms observed in a gene, particular types of SNPs, or only the rare variation. Furthermore it facilitates tests of meaningful parameters (such as mean derived allele burden) and group differences (such as nonsynonymous versus noncoding). The regression toolbox allows model checking and exploration, such as in Figure 3 which presents the data in an informative format. Additional model checking proceeds as usual in linear mixed models, and posterior predictive checks are similarly possible.
A relevant question is how important our method will be for diseases which have not been strongly selected against. There are three answers to consider. First, when selection and disease effect are completely independent, common SNPs will tend to have just as large effect sizes as rare SNPs and explain much of the heritable variation in phenotype [2], [3]. We believe that most investigators conducting resequencing studies assume rare variation to have larger effect sizes, since that is the bestjustified scenario for the expense of sequencing. Second, our method allows for this possibility in the form of estimating to be zero and nonzero. As demonstrated in our simulations, the loss of power in adding a single unnecessary parameter to describe many SNPs is small. Third, as discussed in the Introduction and Text S1, direct selection against disease is not a necessary condition for correlation between fitness and phenotype; as long as the disease related gene is under selective pressure in any of its functions, we expect a correlation.
We have planned several extensions to this method. In addition to improved techniques of estimating fitness effects, we need to incorporate evidence for adaptive selection. Signatures of positive selection [47]–[49] can be used to prioritize genes for study which may have been more important in differentiating humans from our ancestors and hence contribute to modern phenotypes. We expect positively selected variants to have very different phenotype effects from neutral alleles, but it is not clear apriori what that relationship should be or if it will be possible to reliably identify positively selected SNPs [50], [51]. Second, for mathematical and numerical convenience we have developed this method in the context of a prospective probability sample measuring a quantitative trait. Both these assumptions need to be relaxed for the setting of most resequencing projects. Disease phenotypes are frequently nonnormal, binary, or censored such as timetoevent from clinical trials, requiring a generalized linear mixed model. The prospective sampling assumption will also require work to relax. Retrospective sampling such as in casecontrol designs and extremephenotypebased sampling [13], [52] is well known to distort random effect distributions [53]. Third, in our example and simulations, we assume that are independent of one another, but one need not do this. One could add spatial covariance structures between to relax the independence assumption, which would correspond to allowing that variants nearby each other in the genome or folded protein tend to have similar effects. Especially in exomeonly resequencing studies, consideration of unobserved linked markers with techniques similar to TreeLD [35] will be important. Our model has not included dominance or epistasis between SNPs or genes, the structure of which is probably not simple, although progress has been made on determining the impact of these features to quantitative traits [54], [55]. Finally, because our example dataset comes from highquality Sanger sequencing, we have ignored nonrandom missing data issues. Future work involving second generation sequencing or beyond must address the complex nature of library coverage, alignment error, and genotyping error inherent in those technologies.
Supporting Information
Zdroje
1. MaherB
2008 Personal genomes: The case of the missing heritability. Nature 456 18 21
2. PritchardJK
CoxNJ
2002 The allelic architecture of human disease genes: common diseasecommon variant… or not? Hum Mol Genet 11 2417 2423
3. PritchardJK
2001 Are rare variants responsible for susceptibility to complex diseases? American Journal of Human Genetics 69 124137
4. ManolioTA
CollinsFS
CoxNJ
GoldsteinDB
HindorffLA
2009 Finding the missing heritability of complex diseases. Nature 461 747 753
5. EyreWalkerA
2010 Evolution in health and medicine sackler colloquium: Genetic architecture of a complex trait and its implications for fitness and genomewide association studies. Proceedings of the National Academy of Sciences 107 1752 1756
6. GorlovIP
GorlovaOY
SunyaevSR
SpitzMR
AmosCI
2008 Shifting paradigm of association studies: Value of rare SingleNucleotide polymorphisms. American Journal of Human Genetics 82 100112
7. LiB
LealSM
2009 Discovery of rare variants via sequencing: Implications for the design of complex trait association studies. PLoS Genet 5 e1000481 doi:10.1371/journal.pgen.1000481
8. RomeoS
YinW
KozlitinaJ
PennacchioLA
BoerwinkleE
2009 Rare lossoffunction mutations in ANGPTL family members contribute to plasma triglyceride levels in humans. The Journal of Clinical Investigation 119 70 79
9. PaisnRuizC
WasheckaN
NathP
SingletonAB
CorderEH
2009 Parkinson's disease and low frequency alleles found together throughout LRRK2. Annals of Human Genetics 73 391 403
10. CohenJC
PertsemlidisA
FahmiS
EsmailS
VegaGL
2006 Multiple rare variants in NPC1L1 associated with reduced sterol absorption and plasma lowdensity lipoprotein levels. Proceedings of the National Academy of Sciences of the United States of America 103 1810 1815
11. CohenJC
BoerwinkleE
MosleyTH
HobbsHH
2006 Sequence variations in PCSK9, low LDL, and protection against coronary heart disease. N Engl J Med 354 1264 1272
12. RomeoS
PennacchioLA
FuY
BoerwinkleE
TybjaergHansenA
2007 Populationbased resequencing of ANGPTL4 uncovers variations that reduce triglycerides and increase HDL. Nat Genet 39 513 516
13. KotowskiIK
PertsemlidisA
LukeA
CooperRS
VegaGL
2006 A spectrum of PCSK9 alleles contributes to plasma levels of LowDensity lipoprotein cholesterol. The American Journal of Human Genetics 78 410 422
14. CohenJC
KissRS
PertsemlidisA
MarcelYL
McPhersonR
2004 Multiple rare alleles contribute to low plasma levels of HDL cholesterol. Science 305 869 872
15. WangJ
CaoH
BanMR
KennedyBA
ZhuS
2007 Resequencing genomic DNA of patients with severe hypertriglyceridemia (MIM 144650). Arterioscler Thromb Vasc Biol 27 2450 2455
16. KryukovGV
ShpuntA
StamatoyannopoulosJA
SunyaevSR
2009 Power of deep, allexon resequencing for discovery of human trait genes. Proceedings of the National Academy of Sciences 106 3871 3876
17. RoachJC
GlusmanG
SmitAFA
HuffCD
HubleyR
2010 Analysis of genetic inheritance in a family quartet by WholeGenome sequencing. Science 328 636 639
18. HoggartCJ
WhittakerJC
IorioMD
BaldingDJ
2008 Simultaneous analysis of all SNPs in GenomeWide and ReSequencing association studies. PLoS Genet 4 e1000130 doi:10.1371/journal.pgen.1000130
19. KweeLC
LiuD
LinX
GhoshD
EpsteinMP
2008 A powerful and flexible multilocus association test for quantitative traits. American Journal of Human Genetics 82 386 397
20. LiB
LealS
2008 Methods for detecting associations with rare variants for common diseases: Application to analysis of sequence data. The American Journal of Human Genetics 83 311 321
21. MadsenBE
BrowningSR
2009 A groupwise association test for rare mutations using a weighted sum statistic. PLoS Genet 5 e1000384 doi:10.1371/journal.pgen.1000384
22. BartonNH
KeightleyPD
2002 Understanding quantitative genetic variation. Nat Rev Genet 3 11 21
23. JohnsonT
BartonN
2005 Theoretical models of selection and mutation on quantitative traits. Philosophical Transactions of the Royal Society B: Biological Sciences 360 1411 1425
24. HartlDL
ClarkAG
ClarkAG
1997 Principles of population genetics. Sinauer Sunderland, MA, USA
25. EyreWalkerA
WoolfitM
PhelpsT
2006 The distribution of fitness effects of new deleterious amino acid mutations in humans. Genetics 173 891 900
26. EyreWalkerA
KeightleyPD
2007 The distribution of fitness effects of new mutations. Nat Rev Genet 8 610 618
27. KeightleyPD
EyreWalkerA
2007 Joint inference of the distribution of fitness effects of deleterious mutations and population demography based on nucleotide polymorphism frequencies. Genetics 177 2251 2261
28. WelchJJ
EyreWalkerA
WaxmanD
2008 Divergence and polymorphism under the nearly neutral theory of molecular evolution. Journal of Molecular Evolution 67 418 426
29. KryukovGV
PennacchioLA
SunyaevSR
2007 Most rare missense alleles are deleterious in humans: Implications for complex disease and association studies. American Journal of Human Genetics 80 727739
30. YampolskyLY
KondrashovFA
KondrashovAS
2005 Distribution of the strength of selection against amino acid replacements in human proteins. Hum Mol Genet 14 3191 3201
31. GutenkunstRN
HernandezRD
WilliamsonSH
BustamanteCD
2009 Inferring the joint demographic history of multiple populations from multidimensional SNP frequency data. PLoS Genet 5 e1000695 doi:10.1371/journal.pgen.1000695
32. NielsenR
HubiszMJ
HellmannI
TorgersonD
AndrésAM
2009 Darwinian and demographic forces affecting human protein coding genes. Genome Research 19 838 849
33. BoykoAR
WilliamsonSH
IndapAR
DegenhardtJD
HernandezRD
2008 Assessing the evolutionary impact of amino acid mutations in the human genome. PLoS Genet 4 e1000083 doi:10.1371/journal.pgen.1000083
34. TorgersonDG
BoykoAR
HernandezRD
IndapA
HuX
2009 Evolutionary processes acting on candidate cisRegulatory regions in humans inferred from patterns of polymorphism and divergence. PLoS Genet 5 e1000592 doi:10.1371/journal.pgen.1000592
35. ZollnerS
WenX
PritchardJK
2005 Association mapping and fine mapping with TreeLD. Bioinformatics 21 3168 3170
36. HernandezRD
2008 A flexible forward simulator for populations subject to selection and demography. Bioinformatics 24 2786 2787
37. McCullochCE
SearleSR
2000 Generalized, Linear, and Mixed Models Hoboken, NJ, USA John Wiley & Sons, Inc
38. WedderburnRWM
1974 Quasilikelihood functions, generalized linear models, and the Gauss–Newton method. Biometrika 61 439 447
39. HeydeCC
1997 Quasilikelihood and its application Springer 236
40. LittelRC
MillikenGA
StroupWW
WolfingerRD
1996 SAS system for mixed models SAS Inst
41. R Development Core Team 2009 R: A Language and Environment for Statistical Computing. R Foundation for Statistical Computing, Vienna, Austria. http://www.Rproject.org. ISBN 3900051070
42. VictorRG
HaleyRW
WillettDL
PeshockRM
VaethPC
2004 The dallas heart study: a populationbased probability sample for the multidisciplinary study of ethnic differences in cardiovascular health. The American Journal of Cardiology 93 1473 1480
43. BrowningJD
SzczepaniakLS
DobbinsR
NurembergP
HortonJD
2004 Prevalence of hepatic steatosis in an urban population in the united states: impact of ethnicity. Hepatology (Baltimore, Md) 40 1387 1395
44. hon YauM
WangY
LamKSL
ZhangJ
WuD
2009 A highly conserved motif within the NH2terminal coiledcoil domain of angiopoietinlike protein 4 confers its inhibitory effects on lipoprotein lipase by disrupting the enzyme dimerization. The Journal of Biological Chemistry 284 11942 11952
45. YinW
RomeoS
ChangS
GrishinNV
HobbsHH
2009 Genetic variation in ANGPTL4 provides insights into protein processing and function. The Journal of Biological Chemistry 284 13213 13222
46. MorgenthalerS
ThillyWG
2007 A strategy to discover genes that carry multiallelic or monoallelic risk for common diseases: a cohort allelic sums test (CAST). Mutation Research 615 28 56
47. ZengK
ManoS
ShiS
WuC
2007 Comparisons of site and HaplotypeFrequency methods for detecting positive selection. Mol Biol Evol 24 1562 1574
48. PickrellJK
CoopG
NovembreJ
KudaravalliS
LiJZ
2009 Signals of recent positive selection in a worldwide sample of human populations. Genome Research 19 826 837
49. VoightBF
KudaravalliS
WenX
PritchardJK
2006 A map of recent positive selection in the human genome. PLoS Biol 4 e72 doi:10.1371/journal.pbio.1000072
50. PenningsPS
HermissonJ
2006 Soft sweeps III: the signature of positive selection from recurrent mutation. PLoS Genet 2 e186 doi:10.1371/journal.pgen.0020186
51. PritchardJK
PickrellJK
CoopG
2010 The genetics of human adaptation: hard sweeps, soft sweeps, and polygenic adaptation. Current Biology: CB 20 R208 215
52. AhituvN
KavaslarN
SchackwitzW
UstaszewskaA
MartinJ
2007 Medical sequencing at the extremes of human body mass. American Journal of Human Genetics 80 779 791
53. NeuhausJM
JewellNP
1990 The effect of retrospective sampling on binary regression models for clustered data. Biometrics 46 977 990
54. BartonNH
TurelliM
2004 Effects of genetic drift on variance components under a general model of epistasis. Evolution; International Journal of Organic Evolution 58 2111 2132
55. HillWG
GoddardME
VisscherPM
2008 Data and theory point to mainly additive genetic variance for complex traits. PLoS Genet 4 e1000008 doi:10.1371/journal.pgen.1000008
Štítky
Genetika Reprodukční medicínaČlánek vyšel v časopise
PLOS Genetics
2010 Číslo 11
Nejčtenější v tomto čísle
Tomuto tématu se dále věnují…
 GenomeWide Association Study Identifies Two Novel Regions at 11p15.5p13 and 1p31 with Major Impact on AcutePhase Serum Amyloid A
 The Parental NonEquivalence of Imprinting Control Regions during Mammalian Development and Evolution
 CTCFDependent Chromatin Bias Constitutes Transient Epigenetic Memory of the Mother at the Imprinting Control Region in Prospermatogonia
 Nucleolin Is Required for DNA Methylation State and the Expression of rRNA Gene Variants in