Molecular genotyping of Streptococcus agalactiae isolates with non-typeable serotype, Czech Republic, 2008–2020

Česká verze

Authors: S. Vohrnová ^1,2 ; J. Kozáková ¹
Authors place of work: Národní referenční laboratoř pro streptokokové nákazy, Oddělení bakteriálních vzdušných nákaz, Centrum epidemiologie, a mikrobiologie, Státní zdravotní ústav, Praha ¹; 3. lékařská fakulta Univerzity Karlovy, Praha ²
Published in the journal: Epidemiol. Mikrobiol. Imunol. 71, 2022, č. 4, s. 203-207
Category: Krátké sdělení

Summary

The NRL for Streptococcal Infections performed serotyping of 1038 isolates of Streptococcus agalactiae from 972 patients by the latex agglutination method in 2008–2020.

Forty-three isolates (4.4%) whose serotyping failed were classified as non-typeable. The aim of the present study was to determine the genotype of these non-typeable isolates using multiplex polymerase chain reaction (mPCR). Genotyping was successful in the entire set of 43 non-typeable isolates. The most common genotype was V (41.9%), followed by Ia (20.9%). The isolates serotyped by latex agglutination were predominantly assigned to serotype III (29.2%) and V (26.2%). Complete data were obtained on the prevalence of S. agalactiae serotypes/genotypes in the Czech Republic in 2008–2020. Monitoring the serotype and genotype distribution of the pathogen is a prerequisite for the introduction of a potential vaccine against S. agalactiae into clinical practice.

Keywords:

Streptococcus agalactiae – genotype – serotype

Zdroje

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