TLC a HPLC separace polohových izomerů alkylesterů kyseliny 2-, 3-, 4- {2-hydroxy-3-[(4--difenylmethyl)piperazin-1-yl]propoxy}fenylkarbamové a jejich lipo-hydrofilní vlastnosti

TLC and HPLC Separation of Positional Isomers of Alkylesters of 2-, 3-, 4 -⁠ {2-Hydroxy-3--[(4-diphenylmethyl)piperazine-1-yl]propoxy}phenylcarbamic Acid and Their Lipo-HydrophilicProperties

The paper deals with chromatographic separations of newly prepared substances, aryloxyaminopropanolderivatives. The derivatives represent three homological series of four carbons and fourgroups of positional isomers (methyl -⁠ to butyl -⁠ in positions 2-, 3, and 4-). Thin-layer adsorptionchromatography employed the foil Silufol® UV 254 as the stationary phase and partition chromatography,commercially produced glass plates DC Fertigplatten MERCK RP-8 F254 S. High-performanceliquid chromatography was used to separate positional isomers on the column SupelkosilTMABZ+PLUS. The mobile phase was methanol and acetonitrile in graded rations with water andvarious flow rates of the mobile phase were tested. Partition chromatography, determination ofpartition coefficient in the system octanol-water, and the values of capacity factors k’ of thesubstances was employed to evaluate their lipophilicity.

Key words:
HPLC –⁠ TLC –⁠ positional isomers –⁠ aryloxyaminopropanol –⁠ lipophilicity