3D PRINTED HYDROGEL GLUCOSE SENSOR ON ARGON PLASMA ACTIVATED POLYSTYRENE

Stáhnout PDF English version

Autoři: Nenad Krstic ^1,2*; Jens Jüttner ^1*; Achim Müller ³; Monika Knuth ³; Christiane Thielemann ¹
Působiště autorů: BioMEMS Lab, Faculty of Engineering, Technische Hochschule Aschaffenburg, Würzburger Str. 45, 63743 Aschaffenburg, Germany ¹; Department of Chemistry, Faculty of Science and Mathematics, University of Nis, Visegradska 33, 18106 Nis, Serbia ²; EyeSense GmbH, Stockstädter Straße 17, 63762 Großostheim, Germany ³
Vyšlo v časopise: Lékař a technika - Clinician and Technology No. 2, 2020, 50, 45-48
Kategorie: Původní práce
doi: https://doi.org/10.14311/CTJ.2020.2.01

Souhrn

This study presents a proof of principle concept for a two-dimensional bioprinted glucose sensor on Petri dishes that allows for glucose measurements in cell culture medium. To improve bioink adhesion, the polystyrene surfaces of standard Petri dishes are activated with argon plasma, which increases roughness and hydrophilicity. The bioink containing the sensor chemistry—namely fluorescently labeled ConA/Dextran embedded in alginate microbeads—was printed on the activated Petri dishes with an extrusion-based bioprinter. The printed sensor showed good stability and adhesive properties on polystyrene. The glucose concentration was examined using a standard fluorescence microscope with filters adapted to the emission wavelength of the donor and reference dyes. The printed glucose sensor showed high sensitivity and good linearity in a physiologically relevant range of glucose concentrations.

Klíčová slova:

bioprinted sensor – adhesion – Ar plasma – activated polystyrene – glucose measurement

Zdroje

Müller AJ, Knuth M, Nikolaus KS, Krivánek R, Küster F, Hasslacher C, Auffarth GU. Blood glucose self-monitoring with a long-term subconjunctival glucose sensor. Journal of Diabetes Science and Technology, Jan 2013;7(1):24 34. DOI: 10.1177/193229681300700104
Lee H, Hong YJ, Baik S, Hyeon T, Kim DH. Enzyme‐based glucose sensor: from invasive to wearable device. Advanced healthcare materials. 2018 Apr;7(8):1701150. DOI: 10.1002/adhm.201701150
Sharafeldin M, Jones A, Rusling JF. 3D-printed biosensor arrays for medical diagnostics. Micromachines. 2018 Aug;9(8): 394. DOI: 10.3390/mi9080394
Wang J. Glucose biosensors: 40 years of advances and challenges. Electroanalysis: An International Journal Devoted to Fundamental and Practical Aspects of Electroanalysis. 2001 Aug;13(12):983–8. DOI: 10.1002/1521-4109(200108)13:12<983::AID-ELAN983>3.0.CO;2-%23
Makaram P, Owens D, Aceros J. Trends in nanomaterial-based non-invasive diabetes sensing technologies. Diagnostics. 2014 Jun;4(2):27–46. DOI: 10.3390/diagnostics4020027
Badugu R, Lakowicz JR, Geddes CD. Fluorescence sensors for monosaccharides based on the 6-methylquinolinium nucleus and boronic acid moiety: potential application to ophthalmic diagnostics. Talanta. 2005 Feb 15;65(3):762–8. DOI: 10.1016/j.talanta.2004.08.003
Senior M. Novartis signs up for Google smart lens. September 2014;32(9):856. DOI: 10.1038/nbt0914-856
Liu C, Sheng Y, Sun Y, Feng J, Wang S, Zhang J, Xu J, Jiang D. A glucose oxidase-coupled DNAzyme sensor for glucose detection in tears and saliva. Biosensors and Bioelectronics. 2015 Aug 15;70:455–61. DOI: 10.1016/j.bios.2015.03.070
Bandodkar AJ, Wang J. Non-invasive wearable electro-chemical sensors: a review. Trends in biotechnology. 2014 Jul 1;32(7):363–71. DOI: 10.1016/j.tibtech.2014.04.005
Bruen D, Delaney C, Florea L, Diamond D. Glucose sensing for diabetes monitoring: recent developments. Sensors. 2017 Aug; 17(8):1866. DOI: 10.3390/s17081866
Tric M, Lederle M, Neuner L, Dolgowjasow I, Wiedemann P, Wölfl S, Werner T. Optical biosensor optimized for continuous in-line glucose monitoring in animal cell culture. Analytical and bioanalytical chemistry. 2017 Sep 1;409(24):5711–21. DOI: 10.1007/s00216-017-0511-7
Olivo J, Foglia L, Casulli MA, Boero C, Carrara S, De Micheli G. Glucose and lactate monitoring in cell cultures with a wire-less android interface. In2014 IEEE Biomedical Circuits and Systems Conference (BioCAS) Proceedings 2014 Oct 22 (pp. 400–403). IEEE. DOI: 10.1109/BioCAS.2014.6981747
Ballerstadt R, Schultz JS. A fluorescence affinity hollow fiber sensor for continuous transdermal glucose monitoring. Analytical Chemistry. 2000 Sep 1;72(17):4185–92. DOI: 10.1021/ac000215r
Müller AJ, Knuth M, Nikolaus KS, Herbrechtsmeier P. First clinical evaluation of a new long-term subconjunctival glucose sensor. Journal of diabetes science and technology. 2012 Jul; 6(4):875–83. DOI: 10.1177/193229681200600419
Chen L, Hwang E, Zhang J. Fluorescent Nanobiosensors for Sensing Glucose. Sensors. 2018 May;18(5):1440. DOI: 10.3390/s18051440
Jüttner J, Kress L. Functionalized Hydrogel as Glucose Imaging Sensor for in vitro Cell Cultures. International Student Scientific Conference POSTER 2019, Praque, May 23,1–3.
Bitar R, Cools P, De Geyter N, Morent R. Acrylic acid plasma polymerization for biomedical use. Applied Surface Science. 2018 Aug 1;448:168–85. DOI: 10.1016/j.apsusc.2018.04.129
Levif P, Seguin J, Moisan M, Barbeau J. Influence of substrate materials on inactivation of B. atrophaeus endospores in a reduced-pressure argon plasma. Plasma Processes and Polymers, 2011 May 3;8(617–630). DOI: 10.1002/ppap.201000212
Guruvenket S, Rao GM, Komath M, Raichur AM. Plasma surface modification of polystyrene and polyethylene. Applied surface science. 2004 Sep 15;236(1–4):278–84. DOI: 10.1016/j.apsusc.2004.04.033
Smirnov AV, Atkin VS, Gorbachev IA, Grebennikov AI, Sinev IV, Simakov VV. Surface modification of polystyrene thin films by RF plasma treatment. BioNanoScience. 2017 Dec 1; 7(4):680–5. DOI: 10.1007/s12668-017-0407-1