Antiadhesive, antibiofilm and dispersion activity of biosurfactants isolated from Bacillus amyloliquefaciens 3/22

Download PDF Česká verze

Authors: Karolína Englerová; Radomíra Nemcová; Zdenka Bedlovičová; Eva Styková
Published in the journal: Čes. slov. Farm., 2021; 70, 172-178
Category: Původní práce
doi: https://doi.org/https://doi.org/10.5817/CSF2021-5-172

Summary

The aim of this work was to monitor the potential antibiofilm properties of biosurfactants (BS) isolated from Bacillus amyloliquefaciens 3/22 against biofilm formation of the indicator strain Staphylococcus aureus CCM 4223. In this work, the effect of BS 3/22 on biofilm growth during co-incubation, inhibition of biofilm-forming cell adhesion and biofilm dispersion was studied. BS 3/22 inhibited biofilm formation, with its formation decreasing significantly (p < 0.05; p < 0.01; p < 0.001) with increasing BS 3/22 concentration. BS 3/22 also showed antiadhesive activity, which correlated with the concentration used. The dispersing effect of isolated BS 3/22 on a 24-hour biofilm was also detected. BS 3/22 were effective in biofilm dispersion even at lower concentrations compared to antiadhesive activity and inhibition of biofilm formation.

Keywords:

inhibition – biofilm – biosurfactants – dispersion

Zdroje

1. Kim B. S., Kim J. Y. Optimization Using 3 3 Full-Factorial Design for Crude Biosurfactant Activity from Bacillus pumilus IJ-1 in Submerged Fermentation. Microbiol. Biotechnol. Lett. 2020; 48(1), 48–56.

2. Mehjabin J. J., et al. Biosurfactants from Marine Cyanobacteria Collected in Sabah, Malaysia. J. Nat. Prod. 2020; 83(6), 1925–1930.

3. Shekhar S., Sundaramanickam A., Balasubramanian T. Biosurfactant producing microbes and their potential applications: a review. Crit. Rev. Environ. Sci. Technol. 2014; 45, 1522–1554.

4. Kourmentza K., Gromada X., Michael N., et al. Antimicrobial activity of lipopeptide biosurfactants against foodborne pathogen and food spoilage microorganisms and their cytotoxicity. Front. Microbiol. 2021; 11, 3398.

5. Nazareth T. C., Zanutto C. P., Tripathi L., et al. The use of low-cost brewery waste product for the production of surfactin as a natural microbial biocide. Biotechnol. Rep. 2020; 28, e00537.

6. Abdollahi S., Tofighi Z., Babaee T., et al. Evaluation of Anti-oxidant and Anti-biofilm Activities of Biogenic Surfactants Derived from Bacillus amyloliquefaciens and Pseudomonas aeruginosa. Iran. J. Pharm. Sci. 2020; 19(2), 115.

7. Tahaei S. A. S., Stájer A., Barrak I., et al. Correlation between biofilm-formation and the antibiotic resistant phenotype in Staphylococcus aureus isolates: a Laboratory -⁠ Based Study in Hungary and a review of the literature. Infect. Drug Resist. 2021; 14, 1155.

8. Meena K. R., Kanwar S. S. Lipopeptides as the antifungal and antibacterial agents: applications in food safety and therapeutics. Biomed. Res. Int. 2015; 2015, 1–15.

9. Merghni A., Dallel I., Noumi E., et al. Antioxidant and antiproliferative potential of biosurfactants isolated from Lactobacillus casei and their antibiofilm effect in oral Staphylococcus aureus strains. Microb. Pathog. 2017; 104, 84–89.

10. Fiľková A. Štúdium účinku prospešných mikroorganizmov na inhibíciu biofilm tvoriacich patogénov. Diplomová práca. Košice: UVLF 2021.

11. Englerová K., Nemcová R., Maďar M., et al. Morské baktérie rodu Bacillus –⁠ prirodzení producenti lipopeptidových biosurfaktantov. In: Seminár doktorandov venovaný pamiatke akademika Boďu. Vedecké práce doktorandov 2019: zborník zo seminára doktorandov venovaného pamiatke akademika Boďu. 1. vydanie. Košice: Slovenská akadémia vied, Centrum biovied 2019; 17–19.

12. Płaza G., Chojniak J., Rudnicka K., et al. Detection of biosurfactants in Bacillus species: Genes and products identification. J. Appl. Microbiol. 2015; 119, 1023 –⁠ 1034.

13. O’Toole G. A., Pratt L. A., Watnick P. I., et al. Genetic approaches to study of biofilms. In: Methods in enzymology. Elsevier 1999; 91–109.

14. Madhu A. N., Prapulla, S. G. Evaluation and functional characterization of a biosurfactant produced by Lactobacillus plantarum CFR 2194. Appl. Biochem. Biotechnol. 2014; 172(4), 1777–1789.

15. e Silva S. S., Carvalho J. W. P., Aires C. P., et al. Disruption of Staphylococcus aureus biofilms using rhamnolipid biosurfactants. Int. J. Dairy Sci. 2017; 100(10), 7864–7873.

16. Cochrane S. A., Vederas J. C. Lipopeptides from Bacillus and Paenibacillus spp.: a gold mine of antibiotic candidates. Med. Res. Rev. 2016; 36(1), 4–31.

17. Landman D., Georgescu C., Martin D. A., et al. Polymyxins revisited. Clin. Microbiol. Rev. 2008; 21, 449–465.

18. Vater J., Kablitz B., Wilde C., et al. Matrix-assisted laser desorption ionization–time of flight mass spectrometry of lipopeptide biosurfactants in whole cells and culture filtrates of Bacillus subtilis C-1 isolated from petroleum sludge. Appl. Environ. Microbiol. 2002; 68, 6210–6219.

19. Naruse N., Tenmyo O., Kobaru S., et al. Pumilacidin, a complex of new antiviral antibiotics. Production, isolation, chemical properties, structure and biological activity. J. Antibiot. 1990; 43, 267–280.

20. Grangemard I., Wallach J., Maget-Dana R., et al. Lichenysin: A more efficient cation chelator than surfactin. Appl. Biochem. Biotechnol. 2001; 90, 199–210.

21. Saini H. S., Barragán-Huerta B. E., Lebrón-Paler A., et al. Efficient purification of the biosurfactant viscosin from Pseudomonas libanensis strain M9-3 and its physicochemical and biological properties. J. Nat. Prod. 2008; 71, 1011–1015.

22. Benincasa M., Abalos A., Oliveira I., et al. Chemical structure, surface properties and biological activities of the biosurfactant produced by Pseudomonas aeruginosa LBI from soapstock. Antonie Van Leeuwenhoek 2004; 85, 1–8.

23. Díaz De Rienzo M. A., Banat I. M., Dolman B., et al. Sophorolipid biosurfactants: Possible uses as antibacterial and antibiofilm agent. New Biotechnol. 2015; 32, 720–726.

24. Kitamoto D., Yanagishita H., Shinbo T., et al. Surface active properties and antimicrobial activities of mannosylerythritol lipids as biosurfactants produced by Candida antarctica. J. Biotechnol. 1993; 29, 91–96.

25. Abdelli F., Jardak M., Elloumi J., et al. Antibacterial, anti-adherent and cytotoxic activities of surfactin(s) from a lipolytic strain Bacillus safensis F4. Biodegradation 2019; 30, 287–300.

26. Moryl M., Spętana M., Dziubek K., et al. Antimicrobial, antiadhesive and antibiofilm potential of lipopeptides synthesised by Bacillus subtilis, on uropathogenic bacteria. Acta Biochim. Pol. 2015; 62(4).

27. Liu J., Li W., Zhu X., et al. Surfactin effectively inhibits Staphylococcus aureus adhesion and biofilm formation on surfaces. Appl. Microbiol. Biotechnol. 2019; 103, 4565–4574.

28. Ahimou F., Jacques P., Deleu M. Surfactin and iturin A effects on Bacillus subtilis surface hydrophobicity. Enzyme Microb. Technol. 2000; 27(10), 749–754.

29. Janek T., Łukaszewicz M., Krasowska A. Antiadhesive activity of the biosurfactant pseudofactin II secreted by the Arctic bacterium Pseudomonas fluorescens BD5. BMC Microbiol. 2012; 12(1), 1–9.

30. de Araujo L. V., Guimarães C. R., e Silva S,S, Marquita R. L., et al. Rhamnolipid and surfactin: Anti-adhesion/ antibiofilm and antimicrobial effects. Food Control 2016; 63, 171–178.

31. Giri S. S., Ryu E. C., Sukumaran V., et al. Antioxidant, antibacterial, and anti-adhesive activities of biosurfactants isolated from Bacillus strains. Microb. Pathog. 2019; 132, 66–72.

32. Meena K. R., Sharma A., Kanwar S. S. Antitumoral and antimicrobial activity of surfactin extracted from Bacillus subtilis KLP2015. Int. J. Pept. Res. Ther. 2020; 26(1), 423–433.

33. Díaz De Rienzo M. A., Stevenson P. S., Marchant R., et al. Pseudomonas aeruginosa biofilm disruption using microbial surfactants. J. Appl. Microbiol. 2016; 120(4), 868–876.

34. Araujo L. V. D., Freire D. M. G., Nitschke M. Biossurfactantes: propriedades anticorrosivas, antibiofilmes e antimicrobianas. Quim. Nova 2013; 36(6), 848–858.