Viability of the Parathyroid Gland Tissue Measured by Flow Cytometry

Overview

Background.
Postoperative hypoparathyroidism after the total parathyroidectomy (PTX) remains a problem, nomatter our experiences with 243 operations on parathyroid glands (PG). Implantation of „fresh“ tissue, cryopreservationand reimplantation of cryopreserved tissue are performed with uncertain results. The aim of this project wasto compare viability of cryopreserved tissue of parathyroid glands with „fresh“ tissue obtained during parathyreoidectomyand with tissue from cadaverous donors.Methods and Results. Group 1 included 55 cryopreserved samples obtained from 41 patients after PTX (22M, 19F,a mean age of 46±11 years). Average duration of storage in liquid nitrogen was 84±49 months. Group 2 included„fresh“ tissue of PG, harvested during PTX. Viability was measured in different time in samples from 42 patientswith hyperparathyroidism (11M, 31F, a mean age of 55±13 years). Group 3 included tissue of 14 cadaverous donorsobtained during multiorgan harvesting (7M, 7F, a mean age of 31±5 years, WIT 32 min). Viability was measured byflow cytometry with propidium iodide after dissociation of tissue. Evaluation of PG tissue was proven by histology.Average viability in group 1 was 36,9±24,7 %, no correlation with the duration of storage in liquid nitrogen wasfound. Average viability in group 2 was 51,4±24 %. Viability in group 3 was 66,8±32 %. Group 1 vs. group 2 weredifferent with p

Key words:
viability, parathyroid glands, cryopreservation, flow cytometry, parathyreoidectomy, cadaverous donors,hypoparathyroidism.