Role of matrix metalloproteinases in wound healing after congenital cleft lip surgery


Authors: K. Bláha 1;  J. Borský 2;  R. Průša 1;  A. Štekláčová 1;  E. Otoupalová 1;  R. Matějová 1;  M. Pechová 1;  K. Kotaška 1;  T. Dostálová 3
Authors‘ workplace: Ústav klinické biochemie a patobiochemie UK 2. LF a FN Motol, Praha, přednosta prof. MUDr. R. Průša, CSc., Klinika plastické chirurgie FN Královské Vinohrady, Praha , přednosta doc. MUDr. M. Tvrdek, CSc., Dětská stomatologická klinika UK 2. LF a FN 1
Published in: Čes-slov Pediat 2012; 67 (1): 11-14.
Category: Original Papers

Overview

Objective:
The purpose of this study was to compare the aspects of wound healing after cleft lip surgery within one week of age and wound healing after surgery within 2–4 months of age. We examined extracellular matrix proteins involved in wound healing, especially concentrations of matrix metalloproteinases in tissue removed during surgery.

Material and methods:
30 tissue samples (16 boys and 14 girls) were removed during surgery within one week of age 17 or within 2–4 months of age 13. Proteins were extracted in cacodylic buffer for 24 hours in temperature 2–8 °C. Total protein concentrations were examined using modification of Lowry method. Samples were examined using ELISA kit Amersham Biotrak Activity Assay (GE Healthcare UK) for detection of MMP-1, MMP-2, and MMP-3 concentrations.

Results:
MMP-1: early surgery 0.017 ± 0.023 µg/g of protein (mean ± SD), 2–4 months surgery 0.028 ± 0.026 µg/g. MMP-3: early surgery 0.200 ± 0.142 µg/g, 2–4 months surgery 0.155 ± 0.093 µg/g. Concentrations of MMP-2 were after dilution above working range at 12 µg/l in all tested samples.

Conclusion:
No significant differences in concentrations of protein and MMPs, MMP-1 and MMP-3 according to time of surgery were found.

Key words:
matrix metalloproteinases, cleft lip, wound healing


Sources

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Labels
Neonatology Paediatrics General practitioner for children and adolescents

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Czech-Slovak Pediatrics

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