Beta Defensins 2 and 3 in Mucosa of Nasal Polyps in Patients with Chronic Rhinosinusitis and Nasal Polyps with a Cultivation Finding of Staphylococcus Aureus on Nasal Mucosa

Authors: Z. Kuchynková 1;  H. Pácová 1,2;  Z. Balatková 1
Authors‘ workplace: Klinika otorinolaryngologie a chirurgie hlavy a krku 1. LF UK a FN Motol, Katedra otorinolaryngologie IPVZ, Praha ;  přednosta prof. MUDr. J. Betka, DrSc. Ústav pro histologii a embryologii, 1. LF UK, Praha 1;  přednosta doc. MUDr. P. Hach, DrSc. 2
Published in: Otorinolaryng. a Foniat. /Prague/, 57, 2008, No. 2, pp. 74-78.
Category: Original Papers


Chronic sinusitis with nasal polyps is an inflammatory disease, where bacterial infection can participate in etiopathogenesis. Human beta defensins (HBD) are antimicrobial peptides, which represent an important component of inborn mucous immunity.

The study investigated whether HBD 2 and 3 are demonstrable at higher levels in nasal polyps as compared with healthy nasal mucosa.

Before sampling of the nasal polyps, cultivation examination of the secrets from nasal cavity was performed. Samples of healthy nasal mucosa (n = 11) and nasal polyps (n = 49) were processed for light microscopy by fixation in 4% formaldehyde for embedding in paraffin, a portion of the material was frozen in liquid nitrogen at -80°C.

HBD 2 and 3 were detected in paraffin and cryostat sections by immunohistochemical methods by means of three-stage immunoperoxidase technique.

Staphylococcus aureus was the most often cultivated pathogen in patients with nasal polyps. In clinically healthy nasal mucosa the presence of HBD-2 was determined in individual cells of glandular ducts and in ciliary cells. In the polyp material the presence of HBD-2 was demonstrated at higher amounts than in clinically healthy nasal mucosa. HBD-2 was demonstrated in ciliary cells and cellular cytoplasm of the glandular ducts. HBD-3 was demonstrated in ciliary cells of surface epithelium as well as in the cells of glandular ducts in the polyps. The reaction product of proved HBD-2 occurred at extremely high amounts in the cell cytoplasm of surface epithelium as well as in cells of gland ducts in the polyp samples with demonstrated presence of Staphylococcus aureus.

It has become obvious that defensins HBD 2 and 3 occurred more often in the tissue of nasal polyps than in healthy nasal mucosa. HBD 3 was demonstrated at extremely high amount in samples of polyps with demonstrated presence of Staphylococcus aureus.

Key words:
beta defensins, nasal polyps, Staphylococcus aureus.


1. Claeys, S., de Belder, T., Holtappels, G., Gevaert, P., Verhasselt, B., van Cauwenberge, P., Bachert, C.: Human beta-defensins and toll-like receptors in the upper airway. Allergy., 58, 2003, s. 748-753.

2. Claeys, S., Van Hoecke, H., Holtappels, G., Gevaert, P., De Belder, T., Verhasselt, B., Van Cauwenberge, P., Bachert, C.: Nasal polyps in patients with and without cystic fibrosis: a differentiation by innate markers and inflammatory mediators. Clin. Exp. Allergy., 35, 2005, s. 467-472.

3. Cole, A. M., Liao, H. I., Stuchlik, O., Tilan, J., Pohl, J., Ganz, T.: Cationic polypeptides are required for antibacterial activity of human airway fluid. J. Immunol., 169, 2002, s. 6985-6991.

4. Fokkens, W., Lund, V., Mullol, J.: European position paper on rhinosinusitis and nasal polyps group 2007. Rhinol, Suppl., 20, 2007, s. 1-136. Review.

5. Chen, C. I., Schaller-Bals, S., Paul, K. P., Wahn, U., Bals, R.: Beta-defensins and LL-37 in bronchoalveolar lavage fluid of patients with cystic fibrosis. J. Cyst. Fibros. 3, 2004, s. 45-50.

6. Chen, P. H., Fang, S. Y.: Expression of human beta-defensin 2 in human nasal mucosa. Eur Arch. Otorhinolaryngol., 261, 2004, s. 238-241.

7. Kao, C. Y., Chen, Y., Thai, P., Wachi, S., Huang, F., Kim, C., Harper, R. W., Wu, R.: IL-17 markedly up-regulates beta-defensin-2 expression in human airway epithelium via JAK and NF-kappaB signaling pathways. J. Immunol., 173, 2004, s. 3482-3491.

8. Lee, S. H., Kim, J. E., Lim, H. H., Lee, H. M., Choi, J. O.: Antimicrobial defensin peptides of the human nasal mucosa. Ann. Otol. Rhinol. Laryngol., 111, 2002, s. 135-1341.

9. Lin, A., Busaba, N. Y.: Staphylococcus aureus and endoscopic sinus surgery. Cur.r Opin Otolaryngol Head Neck Surg., 14, 2006, s.19-22. Review.

10. Menendez, A., Brett Finlay, B.: Defensins in the immunology of bacterial infections. Curr Opin Immunol., 19, 2007, s. 385-391.

11. Ouhara, K., Komatsuzawa, H., Shiba, H., Uchida, Y., Kawai, T., Sayama, K., Hashimoto, K., Taubman, M. A., Kurihara, H., Sugai, M.: Actinobacillus actinomycetemcomitans outer membrane protein 100 triggers innate immunity and production of beta-defensin and the 18-kilodalton cationic antimicrobial protein through the fibronectin-integrin pathway in human gingival epithelial cells. Infect. Immun., 74, 2006, s. 5211-5220.

12. Ramanathan, M. Jr, Lee, W. K., Dubin, M. G., Lin, S., Spannhake, E. W., Lane, A. P.: Sinonasal epithelial cell expression of toll-like receptor 9 is decreased in chronic rhinosinusitis with polyps. Am. J. Rhinol., 21, 2007, s. 110-116.

13. Schibli, D. J., Hunter, H. N., Aseyev, V., Starner, T. D., Wiencek, J. M., McCray, P. B. Jr, Tack, B. F., Vogel, H. J.: The solution structures of the human beta-defensins lead to a better understanding of the potent bactericidal activity of HBD3 against Staphylococcus aureus. J. Biol. Chem., 277, 2002, s. 8279-8289.

14. Singh, P. K., Jia, H. P., Wiles, K., Hesselberth, J., Liu, L., Conway, B. A., Greenberg, E. P., Valore, E. V., Welsh, M. J., Ganz, T., Tack, B. F., McCray, P. B. Jr.: Production of beta-defensins by human airway epithelia. Proc. Natl. Acad. Sci U S A., 95, 1998, s. 4961-4966.

15. Voss, E., Wehkamp, J., Wehkamp, K., Stange, E. F., Schröder, J. M., Harder, J.: NOD2/CARD15 mediates induction of the antimicrobial peptide human beta-defensin-2. J Biol. Chem., 281, 2006, s. 2005-2011.

16. Wehkamp, J., Schmid, M., Stange, E. F.: Defensins and other antimicrobial peptides in inflammatory bowel disease. Curr Opin Gastroenterol., 23, 2007, s. 370-378. Review.

Audiology Paediatric ENT ENT (Otorhinolaryngology)
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