Amniotic fluid heat shock protein 70 concentration in preterm premature rupture of membranes

Authors: M. Kacerovský 1;  J. Tošner 1;  C. Andrýs 2;  M. Drahošová 2;  L. Plíšková 3;  M. Főrstl 4;  H. Hornychová 5
Authors‘ workplace: Lékařská fakulta Hradec Králové, Univerzita Karlova, Praha Porodnická a gynekologická klinika FN, Hradec Králové, přednosta doc. MUDr. J. Tošner, CSc. 1;  Ústav klinické imunologie a alergologie FN, Hradec Králové, přednosta prof. RNDr. J. Krejsek, CSc. 2;  Ústav klinické biochemie a diagnostiky FN, Hradec Králové, přednosta prof. MUDr. V. Palička, CSc. 3;  Ústav klinické mikrobiologie FN, Hradec Králové, přednosta doc. RNDr. V. Buchta, CSc. 4;  Fingerlandův ústav patologie FN, Hradec Králové, přednosta prof. MUDr. A. Ryška, Ph. D. 5
Published in: Čes. Gynek.2009, 74, č. 2 s. 85-91
Category: Original Article


The purpose of this study was to determinate the changes of amniotic fluid HSP 70 concentrations in patiens with preterm premature rupture of the membranes, and in the presence of intraamniotic infection and histological changes of inflammations.

Prospective study.

Department of Obstetrics and Gynecology Medical Faculty Charles University Hradec Králové.

We studied 30 women between 24 and 36 weeks of gestation with preterm premature rupture of the membranes. Samples of amniotic fluid were collected by transabdominal amniocentesis. These patients were divided into 2 groups. In group 1 were patiens with intraamniotic infection. In group 2 were patiens without intraamniotic infection. Among 76% (35/30) patients placenta were collected and assessed for presence or absence acute inflammatory lesions. HSP70 concentration in amniotic fluid were determined using a sensitive and specific diagnostic kit Hsp 70- ELISA kit manufactered Assay Desings, USA.

There was no significant difference in the median amniotic fluid HSP70 concentration between patients with preterm rupture of the membranes with IAI and without IAI (patients with IAI: median 5.12 ng/ml, range 3.01-90.37 ng/ml vs. patients without IAI: median 4.68 ng/ml, range 0.58-84.28 ng/ml; p=0.56). There was no significant difference in the median amniotic fluid HSP70 concentration between patients with preterm rupture of the membranes with presence and absence histological of acute inflammatory lesions in the placenta and membranes(patients with presence: median 6.97 ng/ml, range 2.61-90.37 ng/ml vs. patients with absence: median 4.63 ng/ml, range 0.58-84.28 ng/ml; p=0.68).

Intraamniotic levels HSP70 were not associated with intraamniotic infection and acute inflammatory lessions in the placenta and membranes.

Key words:
HSP70, intraamniotic infection, chorioamnionitis, preterm premature rupture of the membranes.


1. Area, A. Initiation of the immune response by extracellular HSP72. Curr Immunol Rev, 2006, 2, p. 209-215.

2. Bagchi, MK., Tsai, SY., Tsai, MJ., et al. Progesterone enhanced target gene transcription by receptor free of heat shock proteins hsp90, hsp56 and hsp70. Mol Cell Biol, 1991, 11, p. 4998-5004.

3. Beckmann, RP., Mizzen, LE., Welch, WJ. Interaction of Hsp 70 with newly synthesized proteins: Implications for protein folding and assembly. Science, 1990, 248, p. 850-854.

4. Cohen, IR. Autoimmunity to chaperonins in the pathogenesis of arthritis and diabetes. Ann Rev Immunol, 1991, 9, p. 567-589.

5. DeMarko, AM., Beck, CA., Onate, SA., et al. Dimerization of mammalian progesterone receptors occurs in the absence of DNA and is related to the release of the 90-kDA heat shock protein. Proc Natl Acad Sci USA, 1991, 88, p. 72-76.

6. Ding, XZ., Fernandez-Prada, CM., Bhattacharjee, AK., Hoover, DL. Over-expression of hsp-70 inhibits bacterial lipopolysaccharide-induced production of cytokines in human monocyte derived macrophages. Cytokine, 2001, 16, p. 210-219.

7. Divers, MJ., Bulmer, JN., Miller, D., Lilford, RJ. Placental heat shock proteins: No immunohistochemical evidence for a differential stress response in preterm labour. Gynecol Obstet Invest, 1995, 40, p. 236-243.

8. Fleshner, M., Johnson, JD. Endogenous extracellular heat shock protein 72. Int J Hypertermia, 2005, 21, p. 457-471.

9. Fukushima, A., Kahawara, H., Isurugi, C., et al. Changes in serum levels of heat shock protein 70 in preterm delivery and pre-eclampsia. J Obstet Gynaecol Res, 2005, 31, p. 72-77.

10. Gelber, SE., Bongiovanni, AM., Jean-Pierre, C., et al. Antibodies to the 70 kDa heat shock protein in midtrimester amniotic fluid and intraamniotic imunity. Am J Obstet Gynecol, 2007, 197, p. 278e1-278e4. .

11. Gosgrove, JW., Brown, IR. Heat shock protein in mammalian brain and other organs after a physiologically relevant increase in body temperature induced by D-lysergic acid diethylamide. Proc Natl Acad Sci USA, 1983, 80, p. 569-573.

12. Haslbeck, M. Hsps and their role in the chaperone network. Cell Moll Life Sci, 2002, 59, p. 1649-1657.

13. Chaiworapongsa, T., Erez, O., Kusanovic, JP., et al. Amniotic fluid heat shock protein 70 concentration in histologic chorioamnionitis, term and preterm parturition. J Maternal-Fetal Neonatal Med, 21, 7, p. 449-461.

14. Johnson, JD., Fleshner, M. Releasing sinals, secretory pathways, and imunne function of endogenous extracellular heat shock protein 72. J Leukoc Biol, 2006, 79, p. 425-434.

15. Kacerovský, M., Boudyš, L. Předčasný odtok plodové vody a Ureaplasma urealyticum. Čes Gynek, 2008, 78, p. 154-159.

16. Lee, WC., Wen, HC., Chang, CP., et al. Heat shock protein 72 overexpression proteins against hyperthermia, circulatory shock, and cerebral ischemia during heatstroke. J Appl Physiol, 2006, 100, p. 2073-2082.

17. Menon, R., Gerber, S., Fortunato, SJ., Witkinn, SS. Lipopolysacharide stimulation of 70 kiloDalton heat shock protein messenger ribonucleic acid production in cultured human fetal membranes. J Perinat Med, 2001, 29, p. 133-136.

18. Molvarec, A., Rigo, J. jr., Nagy, B., et al. Serum heat shock protein 70 levels are decreased in normal human pregnancy. J Reprod Immunol, 2007, 74, p. 163-169.

19. Ovelgonne, JH., Souren, JE., Wiegant, FA., et al. Relationship between kadmium-induced expression of heat shock genes, inhibition of protein synthesis and cell death. Toxicology, 1995, 99, p. 19-30.

20. Pockley, AG., Bulmer, J., Hanks, BM., Wright, BH. Identification of human heat shock protein 60 and anti-HSP60 antibodies in the peripheral circulation of normal individuals. Cell Stress Chaperones, 1999, 4, p. 29-35.

21. Pockley, AG. Heat shock proteins as regulator of the immunne response. Lancet, 2003, 362, p. 469-476.

22. Pockley, AG., Sheperd, J., Corton, JM. Detection of heat shock protein 70 and anti-Hsp 70 antibodies in the serum of normal individuals. Immunol Invest, 1998, 27, p. 367-377.

23. Polla, BS. A role for heat shock protein in inflammation? Immunol Today, 1988, 9, p. 134-137.

24. Robert, J. Evolution of heat shock protein and imunity. Dev Comp Immunol, 2003, 27, p. 449-464.

25. Romero, R., Gomez, R., Chaiworapongsa, T., et al. The role of infection in preterm labour and delivery. Paediatr Perinatal Epidemiol, 2001, 15, p. 41-56.

26. Ryan, AJ., Flanagan, SW., Moseley, PL. Gisolfi, CV. Acute heat stress protects rats against endotoxin shock. J Appl Physiol, 1992, 73, p. 1517-1522.

27. Singh, R., Kolvraa, S., Rattan, SI. Genetics of human longevity with emphasis on the relevance HSP70 as candidate genes. Front Biosci, 2007, 12, p. 4504-4513.

28. Stewart, GR., Young, DB. Heat shock proteins and the host patogen interaction during bacterial infection. Curr Opin Immunol, 2004, 16, p. 506-510.

29. Thornberry, N., Caspases, A. Key mediators of apoptosis. Chem Biol, 1998, 5, p. 97-103.

30. Ting, LP., Tu, CL., Chou, CK. Insulin induced expression of human heat shock protein gene hsp 70. J Biol Chem, 1989, 264, p. 3404-3408.

31. Tissieres, A., Mitchell, HK., Tracy, UM. Protein synthesis in solivary gland of Drosophila melanogaster. Relation to chromosome puffs. J Mol Biol, 1974, 84, p. 389-398.

32. Todryk, SM., Gough, MJ., Pockley, AG. Facets of heat shock protein 70 shows immunotherapeutic potential. Immunology, 2003, 110, p. 1-9.

33. Tuohimaa, P., Pekki, A., Blauer, M., et al. Nuclear progesterone receptor is mainly heat shock protein 90- free in vivo. Proc Natl Acad Sci USA, 1993, 90, p. 5848-5852.

34. Vabulas, RM., Ahmad-Nejad, P., Ghose, S., et al. HSP70 as endogenous stimulus of the Toll/interleukin 1 receptor signal pathway. J Biol Chem, 2002, 277, p. 107-115.

35. Welch, WJ. Mammalian stress response: Cell fysiology, structure/fiction of stress proteins, and implications for medicine and disease. Physiol Rev, 1992, 72, p. 1063-1081.

36. Williams, KJ. Landgraf, BE., Whiting, NL., Zurlo, J. Correlation betweens the induction of heat shock protein 70 and enhanced viral reactivation in mammalian cells treated with ultraviolet light and heat shock. Cancer Res, 1998, 49, p. 2735-2742.

37. Wu, T., Tanguay, RM. Antibodies against heat shock proteins in environmental stresses and diseases: friend or foe? Cell Stress Chaperones, 2006, 11, p. 1-12.

38. Wu, YR., Wang, CK., Chen, CM., et al. Analysis of heat shock protein 70 gene polymorphisms and the risk of Parkinson@s disease. Hum Genet, 2004, 114, p. 236-241.

39. Yokota, S., Minota, S., Fuji, N. AntiHSP autoantibodies enhance HSP induced pro-inflamatory cytokine production in human monocyt cells via Toll-Like receptors. Int Immunol, 2006, 18, p. 573-580.

40. Zhang, F, Hackett, NR., Lam, G., et al. Green fluorescent protein selectively induces HSP70 up-regulation of COX2 expresion in endothelial cells. Blood, 2003, 102, p. 2115-2121.

41. Ziegert, M., Witkin, SS., Sziller, I., et al. Heat shock proteins and heat shock protein antibody complexes in placenta tissues. Infect Dis Obstet Gynecol, 1999, 7, p. 180-185.

42. Zugel, U., Kaufmann, SH. Immunne response against heat shock proteins in infectious disease. Immunobiology, 1999, 201, p. 22-35.

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