Virulent profile and resistance of Escherichia coli isolated in immunocompromised patients with urosepsis


Authors: M. Kmeťová;  E. Kendrovská;  V. Takáčová;  M. Sabol;  L. Siegfried
Published in: Urol List 2012; 10(4): 37-40

Overview

We have been studying the characteristics of E. coli strains isolated from blood of immunocompromised patients suffering from urosepsis. In each patient (n = 25) a different strain has been tested. In the tested strains we have most often found gene adherence fimA (96%) and pap (56%). From the genes coding toxin production we have found cnf (20%), α-hly (16%), lt (12%), and st (8%) in our set of strains. The incidence of aer and iucC genes supporting the survival of E. coli in host extracellular space and associated with Fe2+ utilization was 84% and 80%, respectively. From the phylogenetic perspective the studied E. coli strains most often belon-ged to phylogenetic groups B2 and D (53% and 32%, respectively). The highest resistance against antibacterial agents we have found against trimethoprim/sulfamethoxazol and ciprofloxacin (32%) and against ampicillin/ /sulbaktam (20%). All the strains were sensitive to piperacillin/tazobactam, colistin, amikacin and meropenem.

Key words:
Escherichia coli - urosepsis - virulence - resistance - phylogenetic group


Sources

1. Wiles TJ, Kulesus RR, Mulvey MA. Origins and virulence mechanisms of uropathogenic Escherichia coli. Exp Molec Pathol 2008; 85(1): 11–19.

2. Jadhav S, Hussain A, Devi S et al. Phylogenetic and genomic diversity of human bacteremic Escherichia coli strains. BMC Genomics 2008; 9: 560–574.

3. Nataro JP, Kaper JB. Diarrheagenic Escherichia coli. Clin Microbiol Rew 2008; 11(1): 142–201.

4. Brzuszkiewicz E, Thürmer A, Schuldes J et al. Genome sequence analyses of two isolates from the recent Escherichia coli outbreak in Germany reveal the emergence of a new pathotype: Entero-Aggregative-Haemorrhagic Escherichia coli (EAHEC). Arch Microbiol 2011; 193(12): 883–889.

5. Oliveira FA, Paludo KS, Arend LN et al. Virulence characteristics and antimicrobial susceptibility of uropathogenic Escherichia coli strains. Genet Mol Res 2011; 10(4): 4114–4125.

6. Li G, Kariyawasam S, Tivendale KA et al. tkt1, loca­ted on a novel pathogenicity island, is prevalent in avian and human extraintestinal pathogenic Escherichia coli. BMC Microbiology 2012; 12: 51–69.

7. Ananias M, Yano T. Serogroups and virulence genotypes of septicaemic E. coli. Brazilian J Med Biol Res 2008; 41(10): 877-883.

8. Kmeťová M, Frankovičová L, Molokáčová M et al. Molekulárno-biologický dôkaz faktorov virulencie a fylogenetických skupín u klinických izolátov Escherichia coli. Zborník príspevkov. Využitie experimentálnych metód pri ochrane a podpore zdravia obyvateľstva, Košice 2010: 241–245.

9. Siegfried L, Kmeťová M. Role of virulence factors in pathogenesis of Escherichia coli infections. Biologia 1997; 52(6): 697–705.

10. Gudiol C, Calatayud L, Garcia-Vidal C et al. Virulence Plasmid Harbored by Uropathogenic Escherichia coli Functions in Acute Stages of Patho­genesis. Infect Immun 2010; 78(4): 1457–1467.

11. Hilali F, Ruimy R, Saulnier P et al. Prevalence of Virulence Genes and Clonality in Escherichia coli Strains That Cause Bacteremia in Cancer Patients. Infect Immun 2000; 68(7): 3983–3889.

12. Köhler DC, Dobrindt U. What defines extraintestinal pathogenic Escherichia coli? Internat J Med Microbiol 2011; 3(1): 642–647.

13. Kmeťová M, Havrišová K, Sabol M et al. Escherichia coli strains isolated from patients with Crohn’s disease and ulcerative colitis. Biologia 2005; 60: 717–721.

14. Čurová K, Kmeťová M, Sabol M et al. Entero­virulent E. coli in inflammatory and noninflamma-tory bowel diseases. Folia Microbiol 2009; 54(1): 81–86.

15. Clermont O, Bonacorsi S, Bingen E. Rapid and simple determination of the Escherichia coli phylogenetic group. Appl Environ Microbiol 2000; 66(10): 4555–4558.

16. Mokady D, Gophna U, Ron E. Virulence factors of septicemic Escherichia coli strains. Internat J Med Microbiol 2005; 295(6–7): 455–462.

17. Worth L, Slavin MA. Bloodstream infections in hae­matology: Risks and new challenges for prevention. Blood Reviews 2009; 23(3): 113–122.

18. Jaureguy F, Landreau L, Passet V et al. Phylo­genetic and genomic diversity of human bacteremic Escherichia coli strains. BMC Genomics 2008; 9: 560–574.

19. Kmeťová M, Frankovičová L, Molokačová M et al. Resistance to antibiotics, phylogeny group and virulence genes in Escherichia coli isolated from blood in critically diseased patients. 7 th World Congress of the Pediatric Infectious Diseases (WSPID), Mel­bourne, Australia 2011, 782.

20. Cooke NM, Smith SG, Kelleher M et al. Major Differences Exist in Frequencies of Virulence Factors and Multidrug Resistance between Community and Nosocomial Escherichia coli Bloodstream Isolates. J Clin Microbiol 2010; 48(4): 1099–1104.

21. Kmeťová M, Frankovičová L, Hrabovský V et al. Vplyv probiotík na virulenciu E. coli izolovaných pri ulceróznej kolitíde. Správy klin mikrobiol 2011; XI: 2–10.

22. Beerepoot MAJ, den Heijer CDJ, Penders J et al. Predictive value of Escherichia coli susceptibility in strains causing asymptomatic bacteriuria for women with recurrent symptomatic urinary tract infections receiving prophylaxis. Clin Microbiol Infect 2012; 18: E84–E90.

Labels
Paediatric urologist Urology
Login
Forgotten password

Don‘t have an account?  Create new account

Forgotten password

Enter the email address that you registered with. We will send you instructions on how to set a new password.

Login

Don‘t have an account?  Create new account