Assessment of theRatio of the D- and L-Form of Aspartic Acid inIntervertebral Disk Tissue as an Indicator of Age

Authors: A. Pilin;  R. Čabala;  F. Pudil;  V. Bencko
Authors‘ workplace: Ústav soudního lékařství a toxikologie 1. LF UK a VFN Praha Katedra analytické chemie PF UK, Praha Fakulta potravinářské a biochemicé technologie VŠCHT, Praha Ústav hygieny a epidemiologie 1. LF UK, Praha
Published in: Čes.-slov. Patol., , 2001, No. 2, p. 24-26


Assessment of age according to racemization coefficient of aspartic acid in dentin is considereda reliable method. Proteins which are isolated from dentin are non-collagenous proteins or collagen. As collagen is one of the most frequent proteins in the human body, the question arises whether the D-form of aspartic acid can be assessed also in other tissues and the racemizationcoefficient used for assessment of age. In the submitted work the author assessed to use the racemization coefficient in the tissue of the intervertebral disk. A lyophilized portion of the annulusfibrosus was hydrolyzed and the amino acids of the hydrolysate were estimated after derivationby gas chromatography on a chiral capillary column. The values of the racemization coefficientindicate limited possibilities to assess age from thus prepared tissue.

Key words:
assessment of age - intervertebral disk - racemization of aspartic acid - chiral aminoacid separation

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Anatomical pathology Forensic medical examiner Toxicology
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