Protein bcl-2 Expression in the Mid-secretory Endometrium after Oral Estrogen-Progesterone Preparation

Authors: I. Oborná 1;  P. Havelka 3;  V. Lichnovský 2;  J. Březinová 1;  H. Fingerová 1
Authors‘ workplace: Gynekologicko-porodnická klinika LF UP a FN, Olomouc, přednosta prof. MUDr. M. Kudela, CSc. 1;  Ústav histologie a embryologie LF UP, Olomouc, přednosta prof. MUDr. V. Lichnovský, DrSc. 2;  Gyn. -por. oddělení Baťovy nemocnice, Zlín, přednosta prim. MUDr. F. Zábranský, CSc. 3
Published in: Ceska Gynekol 2005; 70(1): 62-66
Category: Original Article


To compare the changes of the protein bcl-2 expression, regulatory mechanism in the process of apoptosis, in the secretory endometrium throughout natural and artificial (estrogen-progesterone substitution) cycles in the same group of patients.

A prospective study.

Dept. of Obstetrics and Gynecology, University Hospital Olomouc, Dept. of Histology and Embryology, Faculty of Medicine, Palack˘ University in Olomouc, Dept. of Obstetrics and Gynecology, Bata Hospital, Zlín

Endometrial samples (n=56) were obtained on days 5 and 7 after ovulation, progesterone addition, resp. Patients (n=14) included in the study had regular menstrual cycle, age under 40, BMI range 20–32 and basal FSH level range 5–9 IU/l. The collected samples were processed routinely and bcl-2 was estimated by indirect three level imunohistochemic method.

We demonstrated the bcl-2 expression in all evaluated layers (epithelial surface, stroma, glands) of the mid-secretory endometrium in both cycles. No difference was found in bcl-2 expression between days 5 and 7 in the secretory phase of the natural cycle. In artificial cycles higher bcl-2 expression was found only in epithelial surface (p<0.05) between days 5 and 7. On day 7 higher bcl-2 expression was found in the artificial cycle in the endometrial epithelial surface (p<0.001) as well as in spongy layer (p<0.01) and compact layer (p<0.05) comparing to day 7 of the natural cycle.

The bcl-2 expression in the mid-secretory endometrium is significantly higher in the cycle with estrogen-progesterone substitution comparing to the natural cycle. These changes were more significant in endometrial and glandular epithelium than in stromal cells.

Key words:
protein bcl-2, endometrium, oral estrogen-progesterone substitution

Paediatric gynaecology Gynaecology and obstetrics Reproduction medicine

Article was published in

Czech Gynaecology

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