Analysis of Cyclosporine A by High Performance Liquid Chromatography (HPLC)
Komzáková I. Brozmanová H.; K. Šafarčík; M. S Grundmann
Ústav klinické farmakologie FNsP a Zdravotně sociální fakulta Ostravské Univerzity, Ostrava HIinika nukleární medicíny FNsP, Ostrava-Poruba HIinika farmakologie Lékařské fakulty Univerzity Palackého, Olomouc
Čes. slov. Farm., 2002; , 107-111
Retem analytical possibilities of therapeutic drog monitoring (TDM) of cyclosporine A (CyA) both in soud organ transplanted patients and in patients with autoimmune diseases are described. The standard method for determination of CyA in blond is a validated HPLC method. HPLC methds esere developed which make it possible to determine not only the parem drog, bot also the main metabolites of CyA: AMI (M17), AM9 (Ml), and AM4N (M21). Preparation of blond samples, their extractions and purifications are discussed. Chromatography is usually carried on C18 or CN columns by isocratic elution under high temperature (70°C). CyD or CyC is used as the internal standard HPLC-MS method enables unambiguous identification of CyA metabolites after their separation on a chromatographic column and it is used urostly for research purposes only.
cyclosporine A - HPLC - HPLC-MS
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