Use of a Redox Polymers for Insulin Labelling with Technetium ( 99m Tc)

Authors: P. Komárek;  I. Kleisner 1;  M. Konopková 2;  I. Komárková 3
Authors‘ workplace: Katedra farmaceutické technologie a kontroly léčiv IPVZ, Praha 1 Klinika nukleární medicíny FNKV, Praha 2 Oddělení radioizotopové diagnostiky IKEM, Praha 3 Oddělení radiofarmak SÚKL, Praha
Published in: Čes. slov. Farm., 1999; , 218-220


The paper describes a method for porcine insulin labelling with technetium ( 99m Tc). When preparingthis peptide, an insoluble redox polymer on the basis of cross-linked dextran (RP G25-IDA) was usedfor labelling. The prepared kits were labelled with 99m Tc with a high labelling efficiency (more than95 %) and stability, as assessed using methods of chromatography. 99m Tc-insulin biodistribution inrats with experimentally induced diabetes – compared with a control group – did not demonstratespecific accumulation of the labelled substance in any organ. The method for kit preparation andits labelling are mild, HPLC and SEC did not reveal any products of insulin degradation and insulincontent met the standards of Ph.B. 97. This is the first case of insulin labelling with techne-tium( 99 Tc) and, also, the first use of a redox polymer for this purpose.

Key words:
insulin – redox polymer – technetium ( 99m Tc) – radioassay – biodistribution

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Pharmacy Clinical pharmacology
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