Hydrazones of R- and S-usnic acid were prepared by condensation of the appropriate enantiomer ofusnic acid with hydrazides of aromatic acids. Prepared compounds were characterized by spectralmethods. Reaction kinetic was studied with capillary electrokinetic chromatography, enantiomericpurity of products was checked by capillary electrophoresis in the presence of g-cyclodextrin.Obtained hydrazones were tested as prospective antimitotic agents. The antitumor activity wasscreened in vitro on human tumor cell lines. The overall activity of tested compounds was low,however, the R- enantiomers were more active than their opposite stereoisomers.