Skeletal Muscle Amino Acid Metabolism in Septic Patients – a Pilot Study

Authors: P. Živný;  V. Černý;  H. Živná;  J. Bureš;  V. Palička;  J. Vávrová
Authors‘ workplace: Ústav klinické biochemie a diagnostiky, Fakultní nemocnice, Hradec Králové, přednosta prof. MUDr . Vladimír Palička, CSc. Klinika anesteziologie a resuscitace, Fakultní nemocnice, Hradec Králové, přednosta doc. MUDr. Vladimír Černý, PhD., FCCM Ústav fyzio
Published in: Anest. intenziv. Med., , 2001, č. 6, s. 314-319


Microdialysis of skeletal muscle in patients with and/or without sepsis was performed to assess changes in their interstitial metabolism. Afterinstitutional approval 7 critically ill patients were divided into two groups: group I. (n = 3) patient without sepsis and group II. (n = 4) patients withsepsis. Microdialysis probe CMA 60 (CMA, Microdialysis, Sweden, cut-off approx. 20 kDa) was inserted into musculus quadriceps femoris.Microdialysis with Ringer’s solution (flow rate 0.6 ml/min) was started. Sixty minutes samples were collected within 24 hours. Venous blood sampleswere taken in 4 hours intervals. Selected amino acids (AA) concentration in microdialysate and venous blood were estimated. There were lowerinterstitial concentrations of glutamine, alanine, isoleucine and tyrosine and higher serine concentrations in interstitial space of skeletal muscle inseptic patients in comparison with non-septic patients. It could be concluded that there are some metabolic reasons for these differences in interstitialamino acid concentrations.

Key words:
microdialysis – sepsis – amino acids

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Anaesthesiology, Resuscitation and Inten Intensive Care Medicine
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