The most frequent methods used for DNA methylation analysis


Authors: Marcela Chmelařová;  Vladimír Palička
Authors‘ workplace: Univerzita Karlova v Praze, Lékařská fakulta a FN Hradec Králové, Ústav klinické biochemie a diagnostiky
Published in: Čas. Lék. čes. 2011; 150: 442-445
Category: Review Articles

Overview

There is an important relationship between the degree of DNA methylation and gene activity in cancer. Number of techniques of DNA methylation analysis has been reported. The aim of this study was to review methodologies that can be used to monitoring DNA methylation changes. We reviewed techniques important for monitoring of DNA methylation changes of CpG islands in gene promoter regions. Advantages, disadvantages and potential use of these techniques are described.

Key words:
DNA hypermethylation, bisulfite conversion, MSP, MS-MLPA.


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