Effect of Growth Factor on the Phenotype of Subpopulations and on the Kinetics of CD34⁺ Cells in the Peripheral Blood and in Grafts of Peripheral Stem Cells in Patients with Non-Hodgkin’s Lymphoma Indicated for Autologous Peripheral Blood Stem Cell Transplantation
Authors:
M. Klabusay 1; D. Lysák 2; V. Hrabčáková 1; M. Navrátil 1; P. Čoupek 3; J. Mayer 1
Authors‘ workplace:
Interní hematoonkologická klinika FN, Brno
1; Hematoonkologické oddělení FN, Plzeň
2; Česká geologická služba
3
Published in:
Čas. Lék. čes. 2008; 147: 319-324
Category:
Original Article
Overview
Background.
Peripheral blood stem cells are the preferred source for transplantation of hematopoiesis in patients with non-Hodgkin’s lymphoma. Application of hematopoietic growth factors is a part of the mobilization chemotherapy regimen. Time overlap of the highest leukocyte and CD34⁺ cell count is required for optimal graft collection. Authors analyzed the effect of two growth factors (leridistim and filgrastim) on the kinetics and phenotype of CD34⁺ cells in patients with non-Hodgkin’s lymphoma indicated for autologous peripheral blood stem cell transplantation.
Methods and Results.
Authors analyzed phenotype of CD34⁺ cell subpopulations and their kinetics in peripheral blood and leukapheresis products by flow cytometry during mobilization and graft collection. Statistically significant differences in expression of lineage-committed antigens between growth factors were found (CD3, CD5 – T-lineage, CD56 NK-lineage, CD20 for B-lineage, p < 0.05), as well as for lineage non–specific antigens (CD38, p < 0.05 and CD54, p < 0.01). The most significant divergence was observed between CD34⁺CD19⁺ subpopulations of leridistim and filgrastim stimulated blood and graft (p < 0.001).
Conclusions.
Expression of lineage-committed antigens on CD34⁺ subpopulations between two growth factors was statistically different. Kinetics of CD34⁺ cells during mobilization regimen with leridistim was not superior to filgrastim concerning the quality of graft.
Key words:
hematopoietic stem cells, transplantation, growth factors, CD34⁺ cell subpopulation, kinetics
Sources
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Journal of Czech Physicians
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