Immunohistochemical Findings in CNS at Infant Sudden Death

Overview

In a group of 7 infants suddenly dying at the age up to 1 year a histological and immunohistoche-mical investigation was performed aimed to prove passible chronic hypoxic and latent traumaticchanges. Presence of ubiquitin and neuron-specific enolase (NSE) was detected in axon andneurons. In six suddenly dying infants an immunohistochemical positivity was found with antibo-dies against ubiquitin in brain stem neurons. Antibodies against NSE showed positivity of thewhite matter was lacking in the detection both of ubiquitin and NSE.Among 12 persons dying of craniocerebral injury an intensive NSE immunopositivity was foundin traumatized axons of 7 cases and ubiquitin positivity in 5 cases while brain stem neurons didnot show any ubiquitin positivity. Brain stem neurons at sudden death were strongly NSE positi-ve in 5 case, weakly positive in 3 cases and negative in 4 deceased.The combined proof of ubiquitin and NSE in neurons and axons at infant sudden death appears tohe advantageous because it combines the detection of traumatic changes and chronic hypoxiclesions as well.

Key words:
infant sudden death - immunohistochemistry - neuron - specific enolase - ubiquitin