The new technologies for the analytical examination of the embryonic metabolome and its prospects


Authors: I. Crha 1;  A. Mádr 2;  J. Musilová 2;  Z. Glatz 2;  J. Žáková 1;  P. Ventruba 1
Authors‘ workplace: Gynekologicko-porodnická klinika LF MU a FN Brno, přednosta prof. MUDr. P. Ventruba, DrSc., MBA 1;  Ústav biochemie, Přírodovědecká fakulta a CEITEC, Masarykova univerzita, Brno, vedoucí prof. RNDr. Z. Glatz, CSc. 2
Published in: Čes. Gynek.2012, 77, č. 6 s. 502-506

Overview

Objective:
To review current technologies for the analytical examination of the embryonic metabolome and its perspectives.

Design:
Review article.

Setting:
Department of Gynecology and Obstetrics, Faculty of Medicine, Masaryk University, and University Hospital, Brno, Department of Biochemistry, Faculty of Science and CEITEC, Masaryk University, Brno.

Methods and results:
Nowadays, very sensitive analytical technologies are available. They enable exact measurement of various molecules – products of embryo metabolism during first days of cultivation. The capillary electrophoresis is one of promising method. Recent studies analysed metabolic differences between embryos that result in a pregnancy and those that do not. Amino acid levels, glucose or pyruvate in the embryo culture media were analysed most frequently. However, results of these studies are ambiguous.

Conclusions:
The capillary electrophoresis with contactless conductivity detection may provide a useful data of the embryonic metabolome. A comprehensive analysis of the used culture medium may represent a valuable adjunct to morphological criteria for enhanced rates of implantation and delivery.

Key words:
metabolomics, metabolome analysis, capillary electrophoresis, embryo, assisted reproductive techniques.


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Labels
Paediatric gynaecology Gynaecology and obstetrics Reproduction medicine

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