Semiquantitative Analysis of mRNA Aromatase Expression in Eutopic Endometrium as a Diagnostic Marker of Endometriosis and Estrogen Dependent Diseases


Authors: J. Višňovský 1;  S. Galo 1;  P. Žúbor 1;  J. Hatok 2;  P. Račay 2;  J. Danko 1
Authors‘ workplace: Gynekologicko-pôrodnícka klinika JLF UK a MFN, Martin, prednosta prof. MUDr. J. Danko, CSc. 1;  Ústav klinickej biochémie JLF UK Martin, prednosta prof. MUDr. D. Dobrota, CSc. 2
Published in: Čes. Gynek.2008, 73, č. 4 s. 213-217

Overview

Objective:
To determine clinical benefits of mRNA aromatase expression in eutopic endometrium as a diagnostic marker of endometriosis.

Design:
Prospective clinical trial.

Setting:
Department of Obstetrics and Gynaecology of Jessenius Medical Faculty and Faculty Hospital, Martin.

Methods:
The expression of mRNA aromatase of eutopic endometrium was determined among women who underwent laparoscopy or laparotomy due to pelvic pain, infertility or benign pelvic tumor. Endometriosis was confirmed histologicaly and classified by rAFS.

Results:
On the basis of entering criteria 23 women were enrolled in this study and divided into two subgroups: 12 endometriotic and 11 without endometriosis. Sensitivity of aromatase expression was 75% and specificity 54,5% at the cut-off value of at least minimal aromatase activity. By the presence of estrogen-dependent diseases- endometriosis, myomas or endometrial hyperplasia 18 women were compared to 5 disease free women. In this case, sensitivity of aromatase expression was 72,2 and specificity 80%.

Conclusion:
Aromatase expression in eutopic endometrium is a good diagnostic marker for endometriosis.

Key words:
endometriosis, adenomyosis, aromatase


Sources

1. Abbott, JA., Hawe, J., Clayton, RD., Garry, R. The effects and effectiveness of laparoscopic excision of endometriosis: a prospective study with 2-5 year follow-up. Hum Reprod, 2003, 18, 9, p.1922-1927.

2. Bhatt, S., Kocakoc, E., Dogra, VS. Endometriosis: sonographic spectrum, Ultrasound Q, 2006, 22, 4, p. 273-280.

3. Bulun, SE., Zeitoun, K., Takayama, K., et al. Estrogen production in endometriosis and use of aromatase inhibitors to treat endometriosis. Endocrine- Related Cancer, 1999, 6, p. 293-301.

4. Bulun, SE., Zeitoun, KM., Takayama, K., Sasano, H. Estrogen biosynthesis in endometriosis: molecular basis and clinical relevance. J Molec Endocrin, 2000, 25, p. 35-42.

5. Bulun, SE., Yang, S., Fang, Z., et al. Estrogen production and metabolism in Endometriosis. Ann New York Acad Sci, 2002, 955, p. 75-85.

6. Diamond, MP., Osteen, KG. Endometrium and Endometriosis. Blackwell Science, Inc., 1997, 384 p.

7. Fang, Z., Yang, S., Gurates, B., et al. Genetic or enzymatic disruption of aromatase inhibits the growth of ectopic uterine tissue. J Clin Endocr Metab, 2002, 87,p. 3460-3466.

8. Ferrero, S., Remorgida. V. Images in clinical medicine. Endometriosis. N Engl J Med, 2007, 357, 7, suppl.

9. Harada, T., Taniguchi, F., Izawa, M., et al. Apoptosis and endometriosis, Front Biosci, 2007, 12, p. 3140-3151.

10. Kitawaki, J., Kusuki, I., Koshiba, H., et al. Detection of aromatase cytochrome P-450 in endometrial biopsy specimens as a diagnostic test for endometriosis. Fertil Steril, 1999, 72, 6, p. 1100-1106.

11. Lebovic, DI., Mueller, MD., Tailor, RN. Immunobiology of endometriosis. Fertil Steril, 2001, 75, 1, p. 1-10.

12. Nezhat, CR., Berger, GS., Nezhat, FR., et al. Endometriosis: Advanced Management and Surgical Technigues. New York: Spinger-Verlag, 1995, p. 175.

13. Simoens, S., Hummelshoj, L., DęHooghe, T. Endometriosis: cost estimates and methodological perspektive. Hum Reprod Update, 2007, 13, p. 395-404.

14. The Revised American Society for Reproductive Medicine classification of endometriosis, Fertil Steril, 1997, 67, p. 817-821.

15. Velasco, I., Rueda, J., Acién, P. Aromatase expression in endometriotic tissues and cell cultures of patients with endometriosis. Mol Hum Reprod, 2006, 12, 6, p. 377-381.

16. Vercellini, P., Parazzini, F., Oldani, S., et al. Adenomyosis at hysterectomy: a study on frequency distribution and patient characteristics. Hum Reprod, 1995, 10, p.1160-1162.

17. Vercellini, P., Fedele, L., Aimi, G., et al. Association between endometriosis stage, lesion type, patient characteristics and severity of pelvic pain symptoms: a multivariate analysis of over 1000 patients. Hum Reprod, 2007, 22, 1, p. 266-271.

Labels
Paediatric gynaecology Gynaecology and obstetrics Reproduction medicine

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2008 Issue 4

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