Fluorescence and Bioluminescence of the Skin

Authors: V. Resl;  P. Cetkovská;  T. Fikrle
Authors‘ workplace: Kožní klinika LF UK, Plzeň přednosta prof. MUDr. Vladimír Resl, CSc.
Published in: Čes-slov Derm, , 2002, No. 5, p. 221-226


According to Stokes law by absorption of photons from emitting radiation longer wave lengthradiation, fluorescence, is stimulated. The phenomenon of fluorescence resembles others such asphosphorescence, chemi-, tribo-, electro-, lyo-, bioluminescence. In dermatology it is used since 1925,Wood’s lamp was discovered in 1903. There exists primary fluorescence of the healthy skin as wellas many pathological processes which are listed in tables and attention is drawn to some lesscommonly used diagnostic possibilities. Frequently also secondary fluorescence is used, i.e. various(incl. intravital) fluorochromes (most frequently fluorescein). This makes possible e.g. the diagnosisof scabies, pigment affections (vitiligo), some contact allergens. There is wide use of immunofluorescentlaboratory and microscopic methods. Last but not least, fluorescence is used for detection ofdrugs, in particular antibiotics (tetracycline, doxycycline) and other chemical substances (dihydroxyacetone).Fluorescence is used also to check the use of preventive preparations for skin protection.It is undergoing brisk development in the therapeutic and diagnostic application of photodynamicallyactive substances in particular porphyrin derivatives. New highly sensitive optoelectronicelements make it possible to introduce spectrofluorimeters into practice.

Key words:
skin fluorescence - Wood’s lamp - primary and secondary fluorescence - applicationof fluorescence - photodynamic diagnosis and therapy - assessment and recording of fluorescence -bioluminescence

Full text is not available online.
If interested in a scan of this journal, contact NTO ČLS JEP.

Dermatology & STDs Paediatric dermatology & STDs
Forgotten password

Don‘t have an account?  Create new account

Forgotten password

Enter the email address that you registered with. We will send you instructions on how to set a new password.


Don‘t have an account?  Create new account