First Pregnancy and Delivery after Cryopreservation ofOocytes in the Czech Republic – Importance of the Method forReproduction Medicine

Authors: R. Hűttelová;  T. Mardešić;  V. Bečvářová;  T. Brachtlová
Authors‘ workplace: Sanatorium Pronatal, Praha, vedoucí lékař doc. MUDr. T. Mardešić, CSc.
Published in: Čes. Gynek.2004, , č. 1 s. 51-55


The purpose of this work was to develop methods for successful cryopreservation ofhuman oocytes.Design: Case-controlled study and case report.Setting: Sanatorium Pronatal, Prague.Methods: Propanediol (PrOH)-sucrose was used as cryoprotectant medium for cumulus-freeoocytes and stepwise dilution of cryoprotectant post-thaw.Results: The method was used in three patients (38 denuded oocytes) and yielded excellent survivaland fertilization rates (89.5% and 73.5% respectively). In all patients embryotransfer waspossible, one patient got pregnant and delivered a healthy baby.Conclusion: Our data show that cryopreservation may ensure that the integrity of the humanoocyte is adequate for normal fertilization and embryo development.

Key words:
infertility, IVF, oocyte, cryopreservation

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Paediatric gynaecology Gynaecology and obstetrics Reproduction medicine

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Czech Gynaecology

2004 Issue 1

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