Authors: V. Eretová; M. Haluzík; J. Svobodová; J. Živný Authors‘ workplace: Gynekologicko-porodnická klinika 1. LF UK a VFN, Praha, přednosta prof. MUDr. J. Živný, DrSc. III. interní klinika 1. LF UK a VFN, Praha, přednosta prof. MUDr. J. Marek, DrSc. Published in: Ceska Gynekol 2002; (1): 20-23 Category:
Objective: To determine whether the blood concentrations of leptin and soluble leptin receptor(LEPR) hang in women after bilateral ovariectomy, and whether there is a relationship betweenthe concentrations of leptin, LEPR and LH, FSH and 17 b-estradiol before and after castration.Design: Prospective clinical study.Setting: Department of Obstetrics and Gynaecology, First Faculty of Medicine, Charles Universityand the General Faculty Hospital, Prague.Methods: 10 women of reproductive age (31–37 years) with a regular menstrual cycle were inclu-ded in the study. Blood samples were always taken during the follicular phase of the menstrualcycle and 5 days after bilateral ovariectomy. In 9 women, hysterectomy was performed with thebilateral ovariectomy. Evaluated parameters: leptin, soluble leptin receptor, FSH, LH, 17 b-estradiol and BMI. Serum soluble leptin receptor and leptin concentrations were determined usingcommercial ELISA kits. Serum concentrations of FSH, LH, and 17 b-estradiol were determinedusing commercial RIA kits.Results: We did not find any statistically significant changes in the concentration of leptin (8.9 ±5.5 vs 7 ± 5.6) and LEPR (16.2 ± 5.6 vs 20.9 ± 8.9) before and after surgical castration. After surgery,according to expectations, the concentration of LH and FSH significantly increased while theconcentration of 17 b-estradiol decreased (P
Key words: leptin, soluble leptin receptor, ovariectomy
Don‘t have an account? Create new account
Enter the email address that you registered with. We will send you instructions on how to set a new password.
