Separation of whole blood and gravity-based manufacturing of blood components by the disable set ErySet® with a hollow-fibre filter

Authors: M. Bohoněk;  M. Petráš;  T. Hrádek;  B. Kostrouchová;  E. Sládková;  M. Kricnerová;  K. Rollerová;  V. Mašková;  S. Duchková
Authors‘ workplace: Oddělení hematologie, biochemie a krevní transfuze, Ústřední vojenská nemocnice – Vojenská fakultní nemocnice Praha
Published in: Transfuze Hematol. dnes,19, 2013, No. 2, p. 88-95.
Category: Comprehensive Reports, Original Papers, Case Reports


The article describes the method of whole blood separation using hollow-fibre filters and the manufacture of leukodepleted red blood cells and plasma using only the force of gravity. It also evaluates the parameters of manufactured blood product quality. The hollow-fibre filter is an integral part of the disposable ErySep® (LMB GmbH, Germany) set, which includes a leukodepletion filter for whole blood filtration. Results from two validation studies are presented. The first study compared the quality parameters of products manufactured using the disposable ErySep® set with the conventional method for manufacturing leukodepleted RBCs and plasma using the classical centrifugation method (Macopharma disposable set Leucoflex with in-line RBCs leukofilter). The second study focused on the quality parameters of blood products manufactured by the new generation of ErySep® disposable set. The results confirm that blood components manufactured using the ErySep® hollow-fibre system have comparable parameters to products manufactured using the standard method. Red cell recovery is even higher. The tested disposable set is suitable for manufacturing high-quality blood products without the additional need for any expensive machines, such as centrifuges, blood separators, etc. This set could be most beneficial for medical facilities where appropriate technical equipment is not available such as field hospitals and in places (or countries) lacking modern infrastructure.

Key words:
separation of whole blood, hollow-fibre filter


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Haematology Internal medicine Clinical oncology
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