Detection of Antibodies to Chlamydophila pneumoniae by Immunoblotting in Patients with Cardiovascular Diseases


Authors: E. Kováčová 1;  M. Mongiellová 2;  J. Tomka 3;  R. Slyško 3;  J. Kazár 2
Authors‘ workplace: Department of Animal Physiology and Ethology, Faculty of natural Sciences, Commenius University, Bratislava 1;  Research Base of the Slovak Medical University, Bratislava 2;  National Instutute of Cardiovascular Diseases, Bratislava 3
Published in: Epidemiol. Mikrobiol. Imunol. 58, 2009, č. 1, s. 15-18

Overview

Chlamydophila pneumoniae, one of the most prevalent human pathogens worldwide, is not only a significant cause of pneumonia, but may also be associated with cardiovascular diseases (CVD) as suggested by multiple studies. A total of 228 sera from CVD patients with hypertension, ischemic heart disease or previous reconstructive vascular surgery were screened for the presence of anti-C. pneumoniae IgG and IgA antibodies by ELISA. Out of 150 positive serum samples, 80 with similar IgG and IgA levels were investigated by immunoblot (IB). IgG antibodies were directed predominantly against the 35 kDa and 39 kDa proteins as well as 50‑54 and 56-60 kDa proteins of C. pneumoniae. IgA antibodies reacted most frequently with the 50-54 and 56-60 kDa proteins.

Key words:
cardiovascular diseases – Chlamydophila pneumoniae antibodies – immunoblotting.


Sources

1. Bas, S., Muzzin, P., Ninet, B., Bornard, J.E. et al. Chlamydial serology: comparative diagnostic value of immunoblotting, microimmunofluorescence test, and immunoassays using different recombinant proteins as antigens. J Clin Microbiol, 2001, 39, 1368-1377.

2. Biendo, M., Eb, F., Lefebvre, J.F., Orfila, J. Limits of the microimmunofluorescence test and advantages of immunoblotting in the diagnosis of chlamydiosis. Clin Diagn Lab Immunol, 1996, 3, 706-709.

3. Blasi, C. The role of the infectious agents in the pathogenesis and evolution of arteriosclerosis. Ann Ital Med Int, 2004, 19, 249-261.

4. Campbell, L.A., Roberts, S., Inoue, S., Kong, L., Kuo, C.-C. Evaluation of Chlamydia pneumoniae 43 and 53 kilodalton recombinant proteins for serodiagnosis by Western Blot. Clin Diagn Lab Immunol, 2001, 8, 1231-1233.

5. Ciervo, A., Vica, P., Petrucca, A., Biasucci, L.M. et al. Antibodies to 60-kilodalton heat shock protein and outer membrane protein 2 of Chlamydia pneumoniae in patients with coronary heart diseases. Clin Diagn Lab Immunol, 2002, 9, 68-74.

6. Essig A., Simnacher U., Susa M., Marre R. Analysis of the humoral immune response to Chlamydia pneumoniae by immunoblotting and immunoprecipitation. Clin Diagn Lab Immunol, 1999, 6, 819-825.

7. Fong, I.V., Chiu, B., Viira, E.R., Tucker, W., Peeling, R.W.. Chlamydia heat shock protein (CHSP-60) antibody and reactive protein (CRP) correlation with Chlamydia pneumoniae in atherosclerotic plaques. J Infect Dis 2002, 186, 1469-1473.

8. Grayston, J.T., Campbell, L.A., Kuo, C.-C., Mordhorst, P. et al. A new respiratory pathogen: Chlamydia pneumoniae strain TWAR. J Infect Dis, 1990, 161, 618-625.

9. Iijima Y., Miyashita N., Kishimoto T., Kanamoto Y. et al. Characterization of Chlamydia pneumoniae species-specific proteins immunodominant in humans. J Clin Microbiol, 1994, 32, 583-588.

10. Jantos, C.A., Heck, S., Roggendorf, R., Sen-Gupta, M., Hegemann, H. Antigenic and molecular analyses of different Chlamydia pneumoniae strains. J Clin Microbiol, 1997, 34, 620-623.

11. Kazár, J., Kovacova E., Koncova K., Cvachova S. et al. Chlamydia pneumoniae antibodies and markers of inflammation in patients with cardiovascular diseases. Bratisl Lek Listy, 2005, 106, 341-344.

12. Kazar, J., Kovacova, E., Mongiellova, V., Gajdos, M. et al. Anti-cytomegalovirus antibodies and other atherosclerosis risk factors in patients with cardiovascular diseases. J Ger Cardiol, 2007, 4, 131-134.

13. Maass M., Giefers J. Cardiovascular disease risk from prior Chlamydia pneumoniae infection can be related to certain antigens recognized in the immunoblot profile. J Infect, 1997, 35, 171-176.

14. Peeling, R.W., Wang, S.-P., Grayston, J.T., Blasi, F. et al. Chlamydia pneumoniae serology: interlaboratory variations in microimmunofluorescence assay results. J Infect Dis, 2000, 181, S426-S429.

15. Saikku, P., Leinonen, M., Mattila, K., Ekman, M.R. et al. Serological evidence of an association of a novel Chlamydia, TWAR, with chronic coronary heart disease and acute myocardial infarction. Lancet, 1988, ii: 983-986.

16. Schumacher, A., Lerkerod, A.B., Seljeflot, I., Sommervoll, L. et al. Chlamydia pneumoniae serology: importance of methodology in patients with coronary heart disease and healthy individuals. J Clin Microbiol, 2001, 39, 1859-1864.

17. Teislerová, D., Zampachová, E. Value of western blotting in serodiagnosis of chlamydial infections (in Czech). Klin Microbiol Infect Lek, 2007, 13, 21-25.

18. Wagels, A.G., Rasmussen, S., Timms, P. Comparison of Chlamydia pneumoniae isolates by western blot (immunoblot) analysis and DNA sequencing of the omp 2 gene. J Clin Microbiol, 1994, 32, 2820-2823.

19. Zeman, K., Pospíšil, L., Čenderle, J., Štroblová, H. et al. Direct and indirect evidence of Chlamydia pneumoniae in patients with significant stenosis of a carotis of atherosclerotic origin. Scripta medica (Brno), 2004, 77, 173-180.

Labels
Hygiene and epidemiology Medical virology Clinical microbiology
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