Polymerase chain reaction: basic principles and applications in molecular pathology

Authors: Libor Staněk
Authors‘ workplace: Ústav patologie 1. LF UK a VFN, Praha
Published in: Čes.-slov. Patol., 49, 2013, No. 3, p. 119-121
Category: Reviews Article


Polymerase chain reaction (PCR) is a technology used for quick and easy amplifying DNA sequences, which is based on the principle of enzymatic replication of the nucleic acids. This method has in the field of molecular biology an irreplaceable role and constitutes one of the basic methods for DNA analysis. In the following article we describe the basic principles of PCR, and its importance especially in the field of pathology.

polymerase chain reaction – nucleic acid amplification – primers – detection of amplicons – reverse transcription


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Anatomical pathology Forensic medical examiner Toxicology
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