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Mediastinal ganglioneuroma with perineural cell differentiation. Report of a case


Authors: M. Zámečník 1;  A. Chlumská 2,3;  F. Ondriaš 1
Authors‘ workplace: Alpha Medical Pathology, s. r. o., Bratislava, Slovak Republic 1;  Šikl`s Department of Pathology, Faculty Hospital, Charles University, Pilsen, Czech Republic 2;  Laboratory of Surgical Pathology, Pilsen, Czech Republic 3
Published in: Čes.-slov. Patol., 48, 2012, No. 2, p. 94-96
Category: Original Articles

Overview

An unusual case of ganglioneuroma with perineural cell differentiation is presented. The tumor was removed from the mediastinum in a 34-year-old male patient. Histologically, it contained neuroid bundles of bland spindle cells, scattered ganglion cells, and some foci of adipocytic metaplasia. Immunohistochemically, the tumor showed expected expressions of S100 protein, neurofilament protein and calretinin. In addition, many spindle cells were positive for perineural cell markers EMA, claudin-1, and GLUT-1. These cells were often arranged in an organoid fashion around the schwannoid bundles. This case indicates that the cells of ganglioneuroma can mature simultaneously towards both Schwann cell and perineural cell phenotypes.

Keywords:
ganglioneuroma – perineurioma – EMA – claudin-1 – GLUT-1


Ganglioneuroma is a benign neoplasm composed of ganglion cells and neuroid spindle cells, occurring usually in adults, and most often located in the posterior mediastinum and retroperitoneum (1). It arises through maturation of neuroblastoma (2,3) or de novo (1). Ultrastructurally, the spindle cell component contains mostly Schwann cells (1). Rare ultrastructural studies have found, in addition to a Schwann cell population, some cells with the features of the perineural cell type (4,5). However, immunohistochemical expression of perineural cell markers such as epithelial membrane antigen (EMA), claudin-1 and GLUT-1 has not yet been described in this type of tumor to our knowledge and according to our literature search. Here, we would like to present a case of ganglioneuroma, in which a well-developed perineural cell component with the expression of perineural cell markers EMA, claudin-1 and GLUT-1 was found (6–8).

MATERIAL AND METHODS

The tissue of the tumor was fixed in 10% formalin and processed routinely. The sections were stained with hematoxylin and eosin. For immunohistochemistry, the following primary antibodies were used: S100 (polyclonal, 1:400), alpha-smooth muscle actin (clone 1A4, 1:1000), desmin (clone D33, 1:3000), neurofilament protein (clone 2F11, 1:1000), GLUT-1 (polyclonal, 1:200), GFAP (polyclonal, 1:3000), EMA (clone E29, 1:700) (all from DAKO, Glostrup, Denmark), calretinin (clone 5A5, 1:100, Novocastra Lab., Newcastle upon Tyne, UK), claudin-1 (polyclonal, 1:50, Zymed, San Francisco, USA), CD34 (clone Qbend/10, 1:800, Novocastra Lab., Newcastle upon Tyne, UK).

Immunostaining was performed according to standard protocols using an avidin-biotin complex labeled with peroxidase or alkaline phosphatase. Appropriate positive and negative controls were applied.

CASE REPORT

In a 34-year-old male patient, a tumor of the posterior mediastinum detected on CT was removed surgically.

Grossly, the tumor tissue was obtained in four fragments which measured together 7.5x5x4cm. The fragments from the marginal zone of the tumor showed a thin capsule. The cut surface of the tissue was grayish-white and fibrous in appearance.

Histologically (Fig. 1), the tumor was composed of irregular neuroid bundles of bland spindle cells with elongated or wavy nuclei and without visible nucleoli. Throughout this neuromatous background, easily visible ganglion cells were scattered. They were seen as isolated cells or in small cell clusters. Some of the ganglion cells were binucleated, and some of them showed degenerative vacuolization or calcification. A few larger interstitial dystrophic calcifications were seen in the tumor. In addition the lesion contained some areas of adipocytic metaplasia that comprised 10 % of the tumor volume. Melanin pigment was not observed in the tumor cells.

Fig. 1. Ganglioneuroma with perineural cell differentiation. The tumor shows typical neuroid stroma and ganglion cells (HE, original magnifications x60 (A) and x160 (B)).
Fig. 1. Ganglioneuroma with perineural cell differentiation. The tumor shows typical neuroid stroma and ganglion cells (HE, original magnifications x60 (A) and x160 (B)).

Immunohistochemically (Fig. 2), ganglion cells expressed strongly neurofilament protein, calretinin and S100 protein (Figs. 2A and 2B). The spindle cells were positive for S100 protein and GFAP. Neurofilament protein and calretinin immunoreactions highlighted also numerous axons in the neuroid stroma. In addition to these expected expressions, numerous spindle cells were positive for perineural cell markers EMA, claudin-1 and GLUT-1 (Figs. 2C–E). These cells were often arranged in organoid fashion around the schwannoid bundles. CD34 stained a few spindle cells. Myoid markers actin and desmin were negative.

Fig. 2. Ganglioneuroma with perineural cell differentiation. Immunohistochemical findings: (A) neurofilament protein highlights ganglion cells and axons; (B) positivity of neuroid bundles for S100 protein; (C) subtle EMA positivity of “thin” perineural cells arranged in organoid fashion around the schwannoid bundles; (D) expression of claudin-1; (E) strong GLUT-1 positivity (ABC technique, original magnifications x60, x160, x200, x160, and x200, respectively).
Fig. 2. Ganglioneuroma with perineural cell differentiation. Immunohistochemical findings: (A) neurofilament protein highlights ganglion cells and axons; (B) positivity of neuroid bundles for S100 protein; (C) subtle EMA positivity of “thin” perineural cells arranged in organoid fashion around the schwannoid bundles; (D) expression of claudin-1; (E) strong GLUT-1 positivity (ABC technique, original magnifications x60, x160, x200, x160, and x200, respectively).

DISCUSSION

The morphology and immunophenotype of the present tumor is typical of ganglioneuroma, mature subtype (1). In addition, our immunohistochemical finding of an expression of perineural cell markers indicates the unquestionable presence of perineural cells in the tumor.

The histogenesis of these cells is unclear. For stromal cells in ganglioneuroma, the following three possible origins are considered (5): 1) neuroblastoma cells are capable of differentiating along neuronal and stromal lines; 2) stromal cells are formed by differentiation of ubiquitous (non-neoplastic) mesenchymal cells in response to the formation of neuritic processes; 3) nerve sheath cells in the surrounding tissues are induced to proliferate and migrate into the tumor. We believe that stromal cells of ganglioneuroma can arise from an immature neuroblastoma cell that is capable of differentiating terminally toward various lines, including the perineural cell line seen in the present case. This appears to be analogical with cells of “mixed“ nerve sheath tumors in which such various lines of differentiation have already been described, for example neurofibroma-schwannoma (9), schwannoma-perineurioma, neurofibroma-perineurioma (10), nerve sheath myxoma (11), and neurofibroma with perineural cells (12,13).

In summary, our case indicates that ganglioneuroma can show also perineural cell differentiation with immunohistochemical expressions of corresponding markers. These expressions (especially of EMA) can be unexpected in ganglioneuroma, but they should not alter the diagnosis. The positivity for perineural cell markers indicates that neuroid stroma of ganglioneuroma is more similar to neurofibroma than to schwannoma.

Correspondence address:

M. Zamecnik, MD

Medicyt, s.r.o., lab. Trencin

Legionarska 28, 91171 Trencin, Slovak Republic

tel: +421-907-156629

e-mail: zamecnikm@seznam.cz


Sources

1. Weiss SW, Goldblum JR. Ewing`s sarcoma/PNET tumor family and related lesions. In: Weiss SW, Goldblum JR, eds. Enzinger and Weissęs Soft Tissue Tumors (4th ed). Philadelphia, PA, USA: Mosby Inc.; 2001: 1265–1322.

2. Shimada H, Ambros I, Dehner LP, Hata J, Joshi VV, Roald B. Terminology and morphologic criteria of neuroblastic tumors: recommendations by the International Neuroblastoma Pathology Committee. Cancer 1999; 86: 349–363.

3. Ijiri R, Tanaka Y, Kato K, Misugi K, Nishihira H, Toyoda Y, et al. Clinicopathologic study of mass-screened neuroblastoma with special emphasis on untreated observed cases: a possible histologic clue to tumor regression. Am J Surg Pathol 2000; 24: 807–815.

4. Shimada H. Transmission and scanning electron microscopic studies on the tumors of neuroblastoma group. Acta Pathol Jpn 1982; 32: 415–426.

5. Ricci A Jr, Parham DM, Woodruff JM, Callihan T, Green A, Erlandson RA. Malignant peripheral nerve sheath tumors arising from ganglioneuromas. Am J Surg Pathol 1984; 8: 19–29.

6. Theaker JM, Gillett MB, Fleming KA, Gatter KC. Epithelial membrane antigen expression by meningiomas, and the perineurium of peripheral nerve. Arch Pathol Lab Med 1987; 111: 409.

7. Folpe AL, Billings SD, McKenney JK, Walsh SV, Nusrat A, Weiss SW. Expression of claudin-1, a recently described tight junction-associated protein, distinguishes soft tissue perineurioma from potential mimics. Am J Surg Pathol 2002; 26: 1620–1626.

8. Ahrens WA, Ridenour RV 3rd, Caron BL, Miller DV, Folpe AL. GLUT-1 expression in mesenchymal tumors: an immunohistochemical study of 247 soft tissue and bone neoplasms. Hum Pathol 2008; 39: 1519–1526.

9. Feany MB, Anthony DC, Fletcher CD. Nerve sheath tumours with hybrid features of neurofibroma and schwannoma: a conceptual challenge. Histopathology 1998; 32: 405–410.

10. Kazakov DV, Pitha J, Sima R, et al. Hybrid peripheral nerve sheath tumors: Schwannoma-perineurioma and neurofibroma-perineurioma. A report of three cases in extradigital locations. Ann Diagn Pathol 2005; 9: 16–23.

11. Zámečník M, Sedláček T. Nerve sheath myxoma with bidirectional schwannomatous and perineural differentiation. Cesk Patol 2010; 46: 73–76.

12. Erlandson RA. The enigmatic perineurial cell and its participation in tumors and in tumorlike entities. Ultrastruct Pathol 1991; 15: 335–351.

13. Zámečník M., Michal M. Perineurial cell differentiation in neurofibromas. Report of eight cases including a case with composite perineurioma-neurofibroma features. Pathol Res Pract 2001; 197: 537–544.

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