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Rectal suction biopsy in clinical practice


Authors: J. Škvařil;  D. Starý;  J. Tůma;  L. Plánka
Authors‘ workplace: Klinika dětské chirurgie, ortopedie a traumatologie, Fakultní nemocnice Brno přednosta prof. MUDr. L. Plánka, Ph. D.
Published in: Čes-slov Pediat 2016; 71 (4): 208-211.
Category: Original Papers

Overview

Introduction:
In diagnosing of the Hirschsprung's disease in clinical practice, in addition to radiological diagnosing by irrigography, is often used a histological diagnosing. Invasive diagnostics method during excision from the rectum shows us the presence of ganglion cells in the submucosal layer. But there is an alternative minimally invasive method to the classical excision by using a suction probe which takes a sample of adequate mucosa and submucosal layer of rectum.

Material and methods:
At our department „RBI system“ (rectal biopsy system) was put into practice for tissue sampling. The system uses vacuum biopsy where tissue is sucked into a sterile disposable capsule and cut necessarily large sample by embedded sharp knife. By vacuum biopsy we took samples of tissue from 15 patients aged from 2 months to 12 years with problems of constipation in order to clarify and precise diagnosis.

Results:
The presence of ganglion cells or describes the structure of the rectal mucosa and submucosal layers were determined in 13 cases after histological processing. 6 patients in the study group were diagnosed by Hirschsprung's disease. Another 3 patients were diagnosed as insufficient resection of fistula after PSARP operation, 2 patients were diagnosed as stenosis and elongated anal canal (funnel anus) and last 4 patients as other bowel movement disorders.

Conclusion:
The results show that the system of suction biopsy fully replaces the traditional excision of the rectum layer and bring us equivalent results with minimal complications. It is an ideal method for early diagnosis of Hirschsprung disease in the group age 0–4 years and thanks to this method we can easily diagnose the cause of constipation and differentiate the surgical cause from other causes of constipation.

Key words:
rectal suction biopsy, Hirschsprung's disease, diagnosing


Sources

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Labels
Neonatology Paediatrics General practitioner for children and adolescents
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