Diffuse Reflectance Spectrophotometry II: Practical Applications – a Pilot Study

Authors: K. Soršáková-Trnovská;  V. Hegyi
Authors‘ workplace: Detská dermatovenerologická klinika LFUK a DFNsP v Bratislave prednosta: doc. MUDr. T. Danilla, CSc.
Published in: Čes-slov Derm, 85, 2010, No. 1, p. 21-28
Category: Clinical and laboratory Research


Diffuse reflectance spectrophotometry is a non-invasive method to investigate optical properties of the skin. This method has a wide clinical use in dermatology, especially, in evaluation of erythematous skin diseases, pigmented skin lesions, disorders of pigmentation and skin tests. We used this method to evaluate normal skin and skin with clinical features of vitiligo within a group of children in age from 3 to 18 years and haemangiomas within a group of neonates to 3. year-old infants. We measured reflected visible light from different areas of normal skin, depigmented skin and haemangioma and made a spectral analysis of data acquired.

Examinations with special probes are perfomed very quickly, in a non-invasive manner, and it is possible to repeat them more times without any discomfort or any negative impact on infants. This method helps to range patients into different phototype groups, gives unique opportunity to monitor morphological haemangiomas changes during growth of the infant, estimates amount of chromophores, mainly oxyhaemoglobine in erythematous lesions, melanin in normal skin, lack of this chromophore in hypopigmented and depigmented skin and determines blood perfusion in examined lesions. According to our data obtained by repeated non-invasive spectral analysis in visible light we can predict the future development of haemangiomas in childhood and manage phototherapy of vitiliginous skin.

Key words:
spectrophotometer – chromophores – hemangioma – vitiligo


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