In vitro Diagnostics of Allergic Contact Dermatitis – Examination of Peripheral Blood Mononuclear Cells of Nickel Allergic Patients by the Means of Lymphocyte Transformation Test and ELISA for Determination of Interleukin 5

Authors: D. Buchvald 1;  L. Lundeberg 2
Authors‘ workplace: Detská dermatovenerologická klinika LF UK a DFNsP, Bratislava prednosta doc. MUDr. T. Danilla, CSc. 1;  Unit of Dermatology and Venereology, Department of Medicine, Karolinska University Hospital, Stockholm, Švédsko prednosta prof. Mona Stahle-Backdahl, MD, PhD. 2
Published in: Čes-slov Derm, 83, 2008, No. 1, p. 16-24
Category: Clinical and laboratory Research


The diagnostics of the etiology of allergic contact dermatitis (ACD) is currently almost solely based on the results of patch tests, which, however, have several disadvantages. Therefore there is a real need for an alternative diagnostic method. The aim of our study was to characterize the potential utility of two different laboratory methods for in vitro diagnosis of contact nickel hypersensitivity. Peripheral blood mononuclear cells of patients with nickel allergic contact dermatitis and a control group of healthy persons without contact allergy were examined and the characteristics of lymphocyte transformation test and interleukin 5 (IL-5) ELISA test were studied as potential diagnostic methods. Our results suggest that both tests might be suitable for in vitro diagnostics of ACD caused by nickel. However, it is necessary to increase the number of patients enrolled into study to confirm this conclusion.

Key words:
diagnostics – allergic contact dermatitis – lymphocyte transformation test – ELISA – IL-5


1. BASKETTER, D., MENNE, T. Lymphocyte transformation test in patients with allergic contact dermatitis. Contact Dermatitis, 2005, 53 (1), p. 1.

2. BOURKE, JF., BATTA, K., PRAIS, L. et al. The reproducibility of patch tests. Br J Dermatol, 1999, 140 (1), p. 102-105.

3. BREHLER, R., MERK, H. In-vitro-Tests zum Nachweis von Kontaktallergien. Hautarzt, 2005, 56 (12), p. 1141-1143.

4. BRUZE, M., ISAKSSON, M., EDMAN, B. et al. A study on expert reading of patch test reactions: inter-individual accordance. Contact Dermatitis, 1995, 32 (6), p. 331-337.

5. BUCHVALD, D. Indukcia sekrécie interleukínu (IL)-5 pri kontaktnom ekzéme. Derma, 2004, 4 (3), p. 4-8.

6. BUCHVALD, D., BUCHVALDOVÁ, D. Antigény pre ELISA test na sérodiagnostiku infekcie Helicobacter pylori. Cs Epidem, 1993, 42 (1), p. 16-21.

7. BUCHVALD, D., LUNDEBERG, L. Impaired responses of peripheral blood mononuclear cells to nickel in patients with nickel-allergic contact dermatitis and concomitant atopic dermatitis. Br J Dermatol, 2004, 150 (3), p. 484-492.

8. CAVANI, A., ALBANESI, C., TRAIDL, C. et al. Effector and regulatory T cells in allergic contact dermatitis. Trends Immunol, 2001, 22 (3), p. 118-120.

9. CEDERBRANT, K., HULTMAN, P., MARCUSSON, JA. et al. In vitro lymphocyte proliferation as compared to patch test using gold, palladium and nickel. Int Arch Allergy Immunol, 1997, 112 (3), p. 212-217.

10. CHRISTIANSEN, J., FARM, G., EID-FOREST, R. et al. Interferon-γ secreted from peripheral blood mononuclear cells as a possible diagnostic marker for allergic contact dermatitis to gold. Contact Dermatitis, 2006, 55 (2), p. 101-112.

11. DE GROOT, AC. Clinical relevance of positive patch test reactions to preservatives and fragrances. Contact Dermatitis, 1999, 41 (4), p. 224-227.

12. DEVOS, SA., VAN DER VALK, PGM. Epicutaneous patch testing. Eur J Dermatol, 2002, 12 (5), p. 506-513.

13. DIEPGEN, TL., COENRAADS, PJ. Sensitivity, specificity and positive predictive value of patch testing: the more you test, the more you get? ESCD Working Party on Epidemiology. Contact Dermatitis, 2000, 42 (6), p. 315-317.

14. EVERNESS, KM., GAWKRODGER, DJ., BOTHAM, PA. et al. The discrimination between nickel-sensitive and non-nickel-sensitive subjects by an in vitro lymphocyte transformation test. Br J Dermatol, 1990, 122 (3), p. 293-298.

15. EWEN, C., BACA-ESTRADA, ME. Evaluation of interleukin-4 concentration by ELISA is influenced by the consumption of IL-4 by cultured cells. J Interferon Cytokine Res, 2001, 21 (1), p. 39-43.

16. FALSAFI-AMIN, H., LUNDEBERG, L., BAKHIET, M. et al. Early DNA synthesis and cytokine expression in the nickel activation of peripheral blood mononuclear cells in nickel-allergic subjects. Int Arch Allergy Immunol, 2000, 123 (2), p. 170-176.

17. HAGEMANN, T., SCHLUTTER-BOHMER, B., ALLAM, JP. et al. Positive lymphocyte transformation test in a patient with allergic contact dermatitis of the scalp after short-term use of topical minoxidil solution. Contact Dermatitis, 2005, 53 (1), p. 53-55.

18. HINDSEN, M., BRUZE, M., CHRISTENSEN, OB. Individual variation in nickel patch test reactivity. Am J Contact Dermat, 1999, 10 (2), p. 62-67.

19. JAKOBSON, E., MASJEDI, K., AHLBORG, N. et al. Cytokine production in nickel-sensitized individuals analysed with enzyme-linked immunospot assay: possible implication for diagnosis. Br J Dermatol, 2002, 147 (3), p. 442-449.

20. JENSEN, CD., PAULSEN, E., ANDERSEN, KE. Retrospective evaluation of the consequence of alleged patch test sensitization. Contact Dermatitis, 2006, 55 (1), p. 30-35.

21. KANERVA, L., ESTLANDER, T., ALANKO, K. et al. Patch test sensitization to Compositae mix, sesquiterpene-lactone mix, Compositae extracts, laurel leaf, Chlorophorin, Mansonone A, and dimethoxydalbergione. Am J Contact Dermat, 2001, 12 (1), p. 18-24.

22. KRASTEVA, M., KEHREN, J., SAYAG, M. et al. Contact dermatitis II. Clinical aspects and diagnosis. Eur J Dermatol, 1999, 9 (2), p. 144-159.

23. MASJEDI, K., AHLBORG, N., GRUVBERGER, B. et al. Methylisothiazolinones elicit increased production of both T helper (Th)1- and Th2-like cytokines by peripheral blood mononuclear cells from contact allergic individuals. Br J Dermatol, 2003, 149 (6), p. 1172-1182.

24. MERK, HF. Lymphocyte transformation test as a diagnostic test in allergic contact dermatitis. Contact Dermatitis, 2005, 53 (4), p. 246.

25. MINANG, JT., ARESTROM, I., TROYE-BLOMBERG, M. et al. Nickel, cobalt, chromium, palladium and gold induce a mixed Th1- and Th2-type cytokine response in vitro in subjects with contact allergy to the respective metals. Clin Exp Immunol, 2006, 146 (3), p. 417-426.

26. MINANG, JT., TROYE-BLOMBERG, M., LUNDEBERG, L. et al. Nickel elicits concomitant and correlated in vitro production of Th1-, Th2-type and regulatory cytokines in subjects with contact allergy to nickel. Scand J Immunol, 2005, 62 (3), p. 289-296.

27. MOED, H., VON BLOMBERG, M., BRUYNZEEL, DP. et al. Improved detection of allergen-specific T-cell responses in allergic contact dermatitis through the addition of ‘cytokine cocktails’. Exp Dermatol, 2005, 14 (8), p. 634-640.

28. RASANEN, L., TUOMI, ML. Diagnostic value of the lymphocyte proliferation test in nickel contact allergy and provocation in occupational coin dermatitis. Contact Dermatitis, 1992, 27 (4), p. 250-254.

29. RUFF, CA., BELSITO, DV. The impact of various patient factors on contact allergy to nickel, cobalt, and chromate. J Am Acad Dermatol, 2006, 55 (1), p. 32-39.

30. RUSTEMEYER, T., VON BLOMBERG, BME., VAN HOOGSTRATEN, IMW. et al. Analysis of effector and regulatory immune reactivity to nickel. Clin Exp Allergy, 2004, 34 (9), p. 1458-1466.

31. SPIEWAK, R., MOED, H., VON BLOMBERG, BME. et al. Allergic contact dermatitis to nickel: modified in vitro test protocols for better detection of allergen-specific response. Contact Dermatitis, 2007, 56 (2), p. 63-69.

32. SWINDELLS, K., BURNETT, N., RIUS-DIAZ, F. et al. Reflectance confocal microscopy may differentiate acute allergic and irritant contact dermatitis in vivo. J Am Acad Dermatol, 2004, 50 (2), p. 220-228.

33. SZEPIETOWSKI, JC., MCKENZIE, RC., KEOHANE, SG. et al. Atopic and non-atopic individuals react to nickel challenge in a similar way. A study of the cytokine profile in nickel-induced contact dermatitis. Br J Dermatol, 1997, 137 (2), p. 195-200.

34. UTER, W., PFAHLBERG, A., GEFELLER, O. et al. Risk factors for contact allergy to nickel-results of a multifactorial analysis. Contact Dermatitis, 2003, 48 (1), p. 33-38.

35. VON BLOMBERG-VAN DER FLIER, M., VAN DER BURG, CKH., POS, O. et al. In vitro studies in nickel allergy: diagnostic value of a dual parameter analysis. J Invest Dermatol, 1987, 88 (4), p. 362-368.

36. WANG, SY., HSU, ML., TZENG, CH. et al. The influence of cryopreservation on cytokine production by human T lymphocytes. Cryobiology, 1998, 37 (1), p. 22-29.

37. WOLF, R., DAVIDOVICI, B., MARCOS, B. et al. Lymphocyte transformation test in patients with allergic contact dermatitis. Contact Dermatitis, 2005, 53 (4), p. 245.

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