Antibodies against the Chlamydial Heat Shock Protein in Women with Periadnexal Adhesions

Czech version

Authors: I. Crha ¹; L. Pospíšil ²; H. Štroblová ³; P. Ventruba ¹; J. Žáková ¹; Martin Huser ¹
Authors‘ workplace: Gynekologicko-porodnická klinika, Masarykova univerzita, Brno, přednosta prof. MUDr. P. Ventruba, DrSc. ¹; Výzkumný ústav veterinárního lékařství, Brno, ředitel doc. MVDr. M. Toman, CSc. ²; Oddělení klinické mikrobiologie, Fakultní nemocnice, Brno, přednostka MUDr. A. Ševčí ³
Published in: Ceska Gynekol 2006; 71(2): 127-131
Category: Original Article

Overview

Objective:
To compare and analyze IgG antibodies against chlamydial heat shock protein (cHSP60) in the blood serum of women with or without periadnexal adhesions in small pelvis.

Design:
Retrospective study.

Setting:
Department of Gynaecology and Obstetrics, Masaryk University, Brno, Veterinary Research Institute, Brno, Department of Clinical Microbiology, Faculty Hospital Brno.

Methods:
IgG antibodies against cHSP60 in the blood serum were detected by means of recombinant enzyme immunoanalysis (“cHSP60 –⁠ IgG -⁠ rELISA medac”). The presence of antibodies was expressed as +, ++ or +++ pozitive. Laparoscopy according to a standard procedure was performed in 76 women (age 28 ± 4.3 years) from couples with fertility disorders treated in the Centre of Reproductive Medicine, Department of Gynaecology and Obstetrics. Chí-square test (programme Statcalc -⁠ EpiInfo 6) and calculation of OR value were used for statistical analysis.

Results:
Twenty-four women (31.6%) had normal finding in small pelvis. Periadnexal adhesions were detected in 43 (56.6%) patients, hydrosalpinx in 13 cases (17.1%), endometriosis in 27 (35.5%) patients and uterine myomas in 11 (14.5%) women. In the group of 33 women without periadnexal adhesions cHSP-60 antibodies were pozitive in 3 women (9.1%), in 43 women with periadnexal adhesions were pozitive in 17 cases (39.5%). In 13 patients with hydrosalpinx cHSP-60 antibodies were pozitive in 61.5%. In patients with periadnexal adhesions semiquantitative pozitivity +++ was detected in 8 cases (18.6%), 4 of them with hydrosalpinx (30.8%). Statistical analysis found significantly higher presence of cHSP-60 antibodies in women with periadnexal adhesions (OR = 6.54, CI interval = 1.59 –⁠ 37.81, chí-square: p = 0.0028)

Conclusion:
Women with periadnexal adhesions have more frequent and stronger pozitivity of IgG antibodies against chlamydial heat shock protein cHSP60.

Key word:
adhesions, Chlamydia trachomatis, heat shock protein, hydrosalpinx, infertility, assisted reproduction