Embryotransfer after 5-day Cultivation

Overview

Objective:
To find out whether: 1) the blastocysts transfer rises the successfulness of IVF and ET(G/ET) and 2) the 2-blastocyst transfer lowers the incidence of multiple pregnancies (IR).Design: Prospective study of 46 patients involved in a prolonged 5-day cultivation of IVF and ETprogramme.Setting: Centre for Assisted Reproduction, Clinic of Gynecology and Obstetrics, Teaching Hospitaland Palacky University in Olomouc.Methods: Patients with at least 5 acquired embryos were included in the group. The cultivation inmedia commercially produced by Scandinavian IVF Science took 5 days.Results: In 91 % of patients in the observed group (n = 46) morulas or blastocysts were acquired.The IVF and ET success rate after 5-day cultivation and subsequent transfer of two embryos was37 % pregnancies per a started cycle (G/cycle), 40 % pregnancies per an embryotransfer (G/ET)and the implantation rate (IR) was 31 %. Relatively high percentage of double pregnancies (53 %)is alarming. Following parameters were assessed: age, sterility cause, number of previous IVFcycles, stimulation scheme, E2 level on the day of hCG application, number of acquired oocytesand pronuclear stages, percentage of grown blastocysts, the quality of transferred embryos andendometrium thickness on the day of transfer. The only statistically significant difference wasfound in E2 level on the day of hCG application. Pregnant patients had lower levels of estradiol(average value 11.8 ± 4.8 nmol/l) compared to patients who did not become pregnant (18.6 ± 11.9nmol/l).Conclusion: In selected groups of patients who refuse multiple pregnancies only one blastocystshould be transferred.

Key words:
IVF and ET, blastocyst transfer