Biosurfactants and their role in the inhibition of the biofilmforming pathogens
Authors:
Karolína Englerová; Radomíra Nemcová; Eva Styková
Published in:
Čes. slov. Farm., 2018; 67, 107-112
Category:
Original Article
Overview
Resistance of pathogenic bacteria is currently one of the major medical problems. Most microbial infections are based on the formation of biofilms, which are a significant reservoir of pathogens. The aim of this study is to determine the antibiofilm and antimicrobial activity of biosurfactants isolated from intestinal lactobacilli and marine bacteria. Biosurfactants (BS) isolated from the strains L. fermentum 2I3, L. fermentum B2/6, L. reuteri SL16, L. reuteri B6/1, S. luteola 3/22, Brevibacillus sp. 4/9, Brevibacillus sp. 2/30 and B. amyloliquefaciens 1/6K significantly (p < 0.001) inhibited the biofilm formation of S. aureus CCM 3953 and P. mirabilis CCM 7188, with higher inhibition detected in BS of marine bacteria when compared to BS isolated from lactobacilli. The results suggest that the mechanism of the antibiofilm effect of BS isolated from lactobacilli against both the reference strains is the same and it is not the result of their antimicrobial action. In contrast, the mechanism of the antibiotic effect of BS isolated from marine bacteria probably depends on the properties of the indicator strain.
Key words:
biosurfactants • biofilm • pathogens • inhibition
Sources
1. Donlan R. M., Costerton J. W. Biofilms, survival mechanisms of clinically relevant microorganisms. Clin. Microbiol. Rev. 2002; 15, 167–193.
2. Kurtz S., et al. Projections of primary and revision hip and knee arthroplasty in the United States from 2005 to 2030. J. Bone Joint Surg. Am. 2007; 89, 780–785.
3. Hamilton H., Jamieson J. Deep infection in total hip arthroplasty. Can. J. Surg. 2008; 51, 111–117.
4. Fracchia L., et al. Potential therapeutic applications of microbial surface-activecompounds. AIMS Bioeng. 2015; 2, 144–162.
5. Chung P. Y., Toh Y. S. Anti-biofilm agents: recent breakthrough against multi-drug resistant Staphylococcus aureus. Pathog. Dis. 2014; 70, 231–239.
6. Jennings E. M., Tanner R. S. Biosurfactant-producing bacteria found in contaminated and uncontaminated soils. Proceedings of the 2000 Conference on Hazardous Waste Research. 2000; 306, 299–306.
7. Rangarajan V., Ramkrishna S. An inexpensive strategy for facilitated recovery of metals and fermentation products by foam fractionation process. Colloids Surf. B. 2013; 104, 99–106.
8. Gudiña E. J., et al. Antimicrobial and antiadhesive properties of a biosurfactant isolated from Lactobacillus paracasei ssp. paracasei A20. Lett. Appl. Microbiol. 2010; 50(4), 419–424.
9. Morikawa M., Hirata Y., Imanaka T. A study on the structure–function relationship of the lipopeptide biosurfactants. Biochim. Biophys. Acta 2000; 1488, 211–218.
10. O’Toole G. A. et al. Genetic approaches to study of biofilms. Methods Enzymol. 1999; 310, 91–109.
11. Santos D. K. F. et al. Biosurfactants: multifunctional biomolecules of the 21st century. Int. J. Mol. Sci. 2016; 17, 401.
12. Gudiña E. J., Teixeira J. A., Rodrigues L. R. Biosurfactants produced by marine microorganisms with therapeutic applications. Mar. Drugs. 2016; 14, E38.
13. Satpute S. K., et al. Multiple roles of biosurfactants in biofilms. Curr. Pharm. Des. 2016; 22, 429–448.
14. Madhu A. N., Prapulla S. G. Evaluation and functional characterization of a biosurfactant produced by Lactobacillus plantarum CFR 2194. Appl. Biochem. Biotechnol. 2014; 172, 1777–1789.
15. Sharma D., et al. Production and structural characterization of Lactobacillus helveticus derived biosurfactant. The Scientific World J. 2014; 1–9.
16. Moldes A. B., et al. Partial characterization of biosurfactant from Lactobacillus pentosus and comparison with sodium dodecyl sulphate for the bioremediation of hydrocarbon contaminated soil. BioMed. Res. Int. 2013; 1–9.
17. Satpute S. K., et al. Biosurfactants from Lactobacilli species: properties, challenges and potential biomedical applications. J. Basic Microbiol. 2016; 56, 1–19.
18. Walter V., Syldatk C., Hausmann R. Screening concepts for the isolation of biosurfactant producing microorganisms. Adv. Exp. Med. Biol. 2010; 672, 1–13.
19. Płaza G. A., Zjawiony I., Banat I. M. Use of different methods for detection of thermophilic biosurfactant-producing bacteria from hydrocarbon-contaminated and bioremediated soils. J. Petrol. Sci. Eng. 2006; 50, 71–77.
20. Sambanthamoorthy K. et al. Antimicrobial and antibiofilm potential of biosurfactants isolated from lactobacilli against multi-drug-resistant pathogens. BMC Microbiol. 2014; 14(1), 197.
21. Fracchia L., et al. Lactobacillus-derived biosurfactant inhibits biofilm formation of human pathogenic Candida albicans biofilm producers. In: Mendez Vilas A (ed.) Current Research, Technology and Education Topics in Applied Microbiology and Microbial Biotechnology; Spain, 2010; 827–837.
22. Femi-Ola T. O., et al. Isolation and screening of biosurfactant bacteria from soil contaminated with domestic waste water. Brit. J. Environ. Sci. 2015; 3(1), 58–63.
23. Lin S. Biosurfactants: Recent advances. J. Chem. Tech. Biotech. 1996; 66(2), 109–120.
24. Rodrigues L., et al. Biosurfactants: potential applications in medicine. J. Antimicrob. Chemother. 2006; 57(4), 609–618.
25. Ciandrini E., et al. Characterization of biosurfactants produced by Lactobacillus. Appl. Microbiol. Biotechnol. 2016; 100(15), 6767–6777.
26. Moryl M., et al. Antimicrobial, antiadhesive and antibiofilm potential of lipopeptides synthesised by Bacillus subtilis, on uropathogenic bacteria. Acta Biochim. Pol. 2015; 62(4), 725–732.
27. Loiseau C., et al. Surfactin from Bacillus subtilis displays an unexpected anti-Legionella activity. Appl. Microbiol. Biotechnol. 2015; 99(12), 5083–5093.
28. Padmavathi A. R., Pandian S. K. Antibiofilm activity of biosurfactant producing coral associated bacteria isolated from Gulf of Mannar. Indian J. Microbiol. 2014; 54(4), 376–382.
29. Kiran G. S., Sabarathnam B., Selvin J. Biofilm disruption potential of a glycolipid biosurfactant from marine Brevibacterium casei. FEMS Immunol. Med. Microbiol. 2010; 59, 432–438.
30. Hassan S. S., Mohammad F. R. Effect of biosurfactant extracted from Bacillus sp. on biofilm formation by some pathogenic bacteria. World J. Exp. Biosci. 2015; 3, 139–145.
31. Carrillo C., et al. Molecular mechanism of membrane permeabilization by the peptide antibiotic surfactin. Biochim. Biophys. Acta 2003; 1611(1), 91–97.
32. Vesterlund S., et al. Staphylococus aureus adheres to human intestinal mucus but can be displaced by certain lactic acid bacteria. Microbiology 2006; 152, 1819–1826.
33. Walencka E., et al. The Influence of Lactobacillus acidophilus-derived surfactants on staphylococcal adhesion and biofilm formation. Folia Microbiol. 2008; 53, 61–66.
34. Banat I. M., De Rienzo M. A. D., Quinn G. A. Microbial biofilms: Biosurfactants as antibiofilm agents. Appl. Microbiol. Biotechnol. 2014; 98, 9915–9929.
35. Saravanakumari P., Mani K. Structural characterization of a novel xylolipid biosurfactant from Lactococcus lactis and analysis of antibacterial activity against multi-drug resistant pathogens. Bioresour. Technol. 2010; 101(22), 8851–8854.
36. Das P., Mukherjee S., Sen R. Antiadhesive action of a marine microbial surfactant. Colloids Surf. B. 2009; 71(2), 183–186.
37. Bernat P. et al. Lipid composition in a strain of Bacillus subtilis, a producer of iturin A lipopeptides that are active against uropathogenic bacteria. World J. Microbiol. Biotechnol. 2016; 32(10), 157.
38. Das P., Mukherjee S., Sen R. Antimicrobial potential of a lipopeptide biosurfactant derived from a marine Bacillus circulans. J. Appl. Microbiol. 2008; 104, 1675–1684.
39. Kitamoto D., et al. Surface-active propertiesand antimicrobial activities of mannosylerythritol lipids asbiosurfactants produced by Candida antarctica. J. Biotechnol. 1993; 29, 91–96.
40. Singh P., Cameotra S. S. Potential applications ofmicrobial surfactants in biomedical sciences. Trends Biotechnol. 2004; 22, 142–146.
Labels
Pharmacy Clinical pharmacologyArticle was published in
Czech and Slovak Pharmacy
2018 Issue 3
Most read in this issue
- Inflammatory bowel disease: factors involved in pathogenesis
- Pharmacy, pharmacists and drugs in the Terezín ghetto
- Biosurfactants and their role in the inhibition of the biofilmforming pathogens
- Imunomodulační aktivita etanolových extraktů z Galium verum L. herb.