Immunofluorescent Identification of Stem Cells in the Culture of Human DonorCornea

Authors: I. Kozák 1;  J. Rosocha 2;  H. Hrbková 2;  T. Juhás 1
Authors‘ workplace: Očná klinika LF UPJŠ, Košice, prednosta doc. MUDr. T. Juhás, DrSc. 2Združená Tkanivová Banka FNsP a LF UPJŠ, Košice, prednosta MVDr. J. Rosocha, CSc. 1
Published in: Čes. a slov. Oftal., , 2003, No. 3, p. 171-175


The purpose of this studywas to identify limbal stemcells in the culture of humandonor cornea. After standard cultivation procedure we usedmonoclonal antibodyagainst alpha-enolase for identification of stem cells. This antibody serves asa probe for the identification of biochemical marker of stem cells. Using immunofluorescentmicroscopy we identified in specimens -enolase positive cellswhichin vitro but not in situshowed alsosomemorphologic differencesfrom othercellular elements of the culture.

Key words:
limbal stem cells, alpha-enolase, immunofluorescent microscopy

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