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Limitation of the use of saliva as diagnostic fluid in clinical practice


Authors: K. Janšáková 1;  P. Celec 1,2,3;  Ľ. Tóthová 1,4
Authors‘ workplace: Ústav molekulárnej biomedicíny, Lekárska fakulta Univerzity Komenského, Bratislava, Slovensko 1;  Ústav patologickej fyziológie, Lekárska fakulta Univerzity Komenského, Bratislava, Slovensko 2;  Katedra molekulárnej biológie, Prírodovedecká fakulta Univerzity Komenského, Bratislava, Slovensko 3;  Fyziologický ústav, Lekárska fakulta Univerzity Komenského, Bratislava, Slovensko 4
Published in: Klin. Biochem. Metab., 26, 2018, No. 1, p. 21-26

Overview

Saliva as a diagnostic fluid has several benefits such the possibility of non-invasive and repeated collection. Many published studies pointed to a wide range of salivary markers of systemic or oral diseases. Nevertheless, saliva has never reached routine clinical practice, mainly due to the low concentration of measured biomarkers, high technical and biological variability and, thus, low reproducibility of obtained results. A key point is the pre-analytical phase including the preparation of the patient, sampling, processing, and the storage of samples. Interpretation is affected by the lack of a normalization factor. Despite many advantages of saliva, it is necessary to focus on the optimization of analytical and pre-analytical procedures. The standardization of the protocols and of the interpretation of the obtained results is a critical condition for the use of saliva in clinical practice.

Keywords:
analysis of biomacromolecules, salivation, saliva.


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Clinical biochemistry Nuclear medicine Nutritive therapist
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