First experience with non-invasive foetal RH genotyping from maternal peripheral blood in alloimmunised pregnancies

Czech version

Authors: I. Hromadníková ¹; K. Veselá ¹; B. Benešová ²; K. Nekovářová ⁴; D. Dušková ⁵; R. Vlk ³; I. Špálová ³; R. Gerychová ⁶; A. Hakenová ⁸; M. Janoušková ⁹; Z. Rosenbaumová ¹⁰; P. Vlašín ¹¹; A. Vlachová ¹²; P. Smejkal ⁷; V. Palásek ¹³; E. Langrová ¹⁴; E. Rožňáková ²; P. Calda ⁴
Authors‘ workplace: Pediatrická klinika ¹; Krevní banka ²; Gynekologicko-porodnická klinika, 2. LF UK a Fakultní nemocnice Motol, Praha ³; Gynekologicko-porodnická klinika a ⁴; Transfuzní oddělení, VFN a 1. LF UK, Praha ⁵; Gynekologicko-porodnická klinika a ⁶; Oddělení klinické hematologie, Fakultní nemocnice Brno ⁷; Transfúzní oddělení, Fakultní Thomayerova nemocnice, Praha ⁸; Transfuzní oddělení, Nemocnice Karlovy Vary ⁹; Ženská klinika, Masarykova nemocnice v Ústí n. L. ¹⁰; Gynekologická a ultrazvuková ambulance, Centrum prenatální diagnostiky, Brno ¹¹; Transfuzní oddělení, Fakultní nemocnice Královské Vinohrady ¹²; Gynekologicko-porodnické oddělení a ¹³; Hematologicko-transfuzní oddělení, Nemocnice Kladno ¹⁴
Published in: Transfuze Hematol. dnes,11, 2005, No. 1, p. 17-20.
Category: Comprehensive Reports, Original Papers, Case Reports

Overview

In this prospective study, we described our first experience with non-invasive foetal RH genotyping in alloimmunised pregnancies by analysis of DNA extracted from maternal plasma samples by using real-time PCR and primers and probes targeted toward RHD and RHCE genes. We analysed 22 alloimmunised pregnant women (15 anti-D, 5 anti-D+C, 2 anti-E) within 11^th and 33^rd week of pregnancy and correlated the results with serological analysis of cord blood. Non-invasive prenatal foetal RHD and RHCE genotyping analysis of maternal plasma samples was in complete concordance with the analysis of cord blood in all alloimmunised pregnancies. Non-invasive foetal RHD and RHCE genotyping enables the identification of foetuses at risk of haemolytic disease of the newborn. An identification of negative foetuses may exclude the demand of invasive prenatal procedures.

Key words:
foetal DNA, haemolytic disease of the newborn, quantitative real-time PCR, maternal plasma, RHD gene, RHCE gene

Labels

Haematology Internal medicine Clinical oncology

Endothelium and haemostasis

Efficacy of rituximab as consolidation of first – line CHOP regimen in patients with follicular lymphomas

Non-invasive fetal RHc genotyping from maternal peripheral blood

Article was published in

Transfusion and Haematology Today

2005 Issue 1