Authors: I. Hromadníková 1; J. Doucha 3; B. Benešová 2; K. Veselá 1; E. Rožňáková 2; A. Hakenová 4 Authors‘ workplace: Pediatrická klinika 1; Krevní banka 2; Gynekologicko-porodnická klinika, 2. LF UK a Fakultní nemocnice Motol, Praha 3; Transfúzní oddělení, Fakultní Thomayerova nemocnice, Praha 4 Published in: Transfuze Hematol. dnes,11, 2005, No. 1, p. 14-16. Category: Comprehensive Reports, Original Papers, Case Reports
In this prospective study, we described our first experience with non-invasive foetal RHc genotyping by analysis of DNA extracted from maternal plasma samples by using real-time PCR, specific primers and TaqMan probe targeted toward c allele of RHCE gene. We analysed 13 non-alloimmunised and 1 anti-c alloimmunised pregnant woman within 12th and 33rd week of pregnancy and correlated the results with serological analysis of cord blood. Non-invasive prenatal foetal RHc genotyping analysis of maternal plasma samples was in complete concordance with the analysis of cord blood in all pregnancies. Non-invasive foetal RHc genotyping enables the identification of foetuses at risk of haemolytic disease of the newborn. An identification of RHc negative foetuses in anti-c alloimmunized pregnancies may exclude the demand of invasive prenatal procedures.
Key words: foetal DNA, haemolytic disease of the newborn, quantitative real-time PCR, maternal plasma, c allele, RHCE gene
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