Authors: Eva Lousová 1; RNDr. Jana Žáková; Ph. D 1; MUDr. Rastislav Beharka 2; prof. MUDr. Pavel Ventruba; DrSc. 1; prof. MUDr. Dalibor Pacík; CSc. 2; Hana Pochopová 1 Authors‘ workplace: Gynekologicko-porodnická klinika FN a MU Brno 1; Urologická klinika FN a MU Brno 2 Published in: Prakt Gyn 2008; 12(1): 36-37
Introduction: Retrograde ejaculation is a rare cause of male infertility. About 2 % of men are affected by it. In the case of azoospermia, it should be confi rmed or excluded by the post-ejaculatory urine test for spermatozoa. The objective of this study was to fi nd a method for the recovery and preparation of spermatozoa for in vitro fertilisation.
Material and method: Urine was centrifuged and processed using the swim-up after urine alkalinisation, masturbation and urination.
Results: Spermatozoa were obtained and cryopreserved from three successive collections performed in one patient. After fertilisation of 9 oocytes with the ICSI method, 2 embryos were suitable for embryotransfer.
Conclusion: The above technique represents a chance for the recovery of spermatozoa in men with complete retrograde ejaculation.
Key words: retrograde ejaculation – spermatozoa – azoospermia – assisted reproduction
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