Detection of free light chains – A New Method of Diagnostics of Haematological Diseases

Czech version

Authors: J. Mecl; H. Benáková ¹; A. Nohejlová ²; J. Straub ²; T. Zima ¹; I. Špička ²
Authors‘ workplace: Nemocnice Na Homolce, Praha ; Ústav klinické biochemie a laboratorní diagnostiky 1. LF a VFN, Praha ¹; I. interní klinika – klinika hematologie 1. LF a VFN, Praha ²
Published in: Čas. Lék. čes. 2007; 146: 159-162
Category: Original Article

Overview

Background.
To diagnose monoclonal gamopathy, one of the most frequent haematological diseases, we use immunochemical assays, which are based on the detection of paraprotein in serum and/or urine.

Methods and Results.
The most common laboratory assays we use are SPE (serum protein electrophoresis) and IFE (immunofixation electrophoresis). New method represents the detection of free light chain (FLC) in serum. In our study we compared those three methods (SPE, IFE and FLC) from the point of sensitivity of paraprotein detection. For FLC detection was used Freelite system analyzer (Immunotech Beckman Coulter). We examined 51 patients with diagnosis of multiple myeloma, nonHodgkin’s lymphoma, primary amyloidosis and monoclonal gammopathy of undetermined significance.

Conclusions.
Detection of FLC is a valuable method which sometimes could specify diagnosis of MG and make the treatment more accurate.

Key words:
multiple myeloma, detection of paraprotein, free light chains.

Labels

Addictology Allergology and clinical immunology Angiology Audiology Clinical biochemistry Dermatology & STDs Paediatric gastroenterology Paediatric surgery Paediatric cardiology Paediatric neurology Paediatric ENT Paediatric psychiatry Paediatric rheumatology Diabetology Pharmacy Vascular surgery Pain management

Free Cell Human DNA in Body Fluids – Potential for Clinical Applications

New antibiotics, efflux pumps and overcoming resistance

Infection and Systemic Inflammatory Response

Imported Tropical Infections

Liver Diseases of the Infectious Aetiology

Genetic Variability of Cytokines in Relation to the Risk of Post–surgical Complications

Pathogenic Entamoeba histolytica – A Rare Incidence in Persons Microscopically Positive for Cysts in Faeces

Infectious Diseases of Drug Users in the Czech Republic

Chronic Appendicitis – Vanishing or Current Diagnosis?

Incidence of Thyroid Cancer in Slovakia: Extensive Evidence from One Centre in the Context of National Data

Wrist Arthroscopy

Rhodococcus equi Infection in Subjects Infected with Human Immunodeficiency Virus (HIV)