Authors: M. Vraná; M. Dobrovolná; P. Cetkovský; S. Nazarová; H. Vondráčková; P. Sedláček 1 Authors‘ workplace: Ústav hematologie a krevní transfuze, Praha ; Klinika dětské hematologie a onkologie 2. LF UK a FNM, Praha 1 Published in: Čas. Lék. čes. 2006; 145: 126-129 Category: Original Article
Background. The HLA-DPB1 gene is probably one of HLA class II genes affecting the haematopoietic stem cell transplantation. The aim of the study was to analyse the HLA-DPB1 gene and its match/mismatch in patients transplanted from unrelated HSC donors. The PCR-SSP method was used for the typing of the HLA system.
Methods and Results. The cohort consisted of 201 pairs of patient/unrelated HSC donor. Match in HLA-A, -B, -Cw, -DRB1 and -DQB1 (e.g. 10/10 match) was found in 81 pairs. The HLA-DPB1 was tested in them. 18 different HLA-DPB1 alleles were identified in this cohort. Complete match (e.g.12/12) was detected in 3% of the 201 analysed pairs only.
Conclusions. The probability of finding 12/12 matching unrelated HSC donor is limited due to the high percentage of mismatches and inaccessibility of previous HLA-DPB1 results.
Key words: haematopoietic stem cell transplantation, major histocompatibility system, HLA class I and HLA class II genotyping, locus, polymorphism, haplotype.
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