HLA-DPB1 Gene Analysis in Haematopoietic Stem Cell Transplantations
Authors:
M. Vraná; M. Dobrovolná; P. Cetkovský; S. Nazarová; H. Vondráčková; P. Sedláček 1
Authors‘ workplace:
Ústav hematologie a krevní transfuze, Praha
; Klinika dětské hematologie a onkologie 2. LF UK a FNM, Praha
1
Published in:
Čas. Lék. čes. 2006; 145: 126-129
Category:
Original Article
Overview
Background.
The HLA-DPB1 gene is probably one of HLA class II genes affecting the haematopoietic stem cell transplantation. The aim of the study was to analyse the HLA-DPB1 gene and its match/mismatch in patients transplanted from unrelated HSC donors. The PCR-SSP method was used for the typing of the HLA system.
Methods and Results.
The cohort consisted of 201 pairs of patient/unrelated HSC donor. Match in HLA-A, -B, -Cw, -DRB1 and -DQB1 (e.g. 10/10 match) was found in 81 pairs. The HLA-DPB1 was tested in them. 18 different HLA-DPB1 alleles were identified in this cohort. Complete match (e.g.12/12) was detected in 3% of the 201 analysed pairs only.
Conclusions.
The probability of finding 12/12 matching unrelated HSC donor is limited due to the high percentage of mismatches and inaccessibility of previous HLA-DPB1 results.
Key words:
haematopoietic stem cell transplantation, major histocompatibility system, HLA class I and HLA class II genotyping, locus, polymorphism, haplotype.
Labels
Addictology Allergology and clinical immunology Angiology Audiology Clinical biochemistry Dermatology & STDs Paediatric gastroenterology Paediatric surgery Paediatric cardiology Paediatric neurology Paediatric ENT Paediatric psychiatry Paediatric rheumatology Diabetology Pharmacy Vascular surgery Pain managementArticle was published in
Journal of Czech Physicians
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