Authors: A. Tuchyňová; J. Rovenský; S. Blažíčková; Ľ. Rauová; L. Hegyi 1; M. Buc 2 Authors‘ workplace: Výskumný ústav reumatických chorôb, Piešťany Slovenská postgraduálna akadémia, Bratislava 2 Imunologický ústav LF UK, Bratislava Published in: Čas. Lék. čes. 1999; : 696-698 Category:
Background. During physiological ageing changes of the immune system take place at several levels. The objectiveof the submitted work was to compare the ability of spontaneous restoration of selected differentiation antigens onlymphocytes in the peripheral blood stream after previous trypsin treatment in a group of healthy elderly and adultsubjects.Methods and Results. Twenty-four adults were examined (19 - 59 years) and 36 elderly subjects (60 - 90 years).Isolated lymphocytes from the peripheral blood stream were treated with trypsin and then incubated in a cultivationmedium. The authors investigated the capacity of restoration of differentiation antigens CD2, CD4, CD8 andCD45RA.Antigen CD2 was not restored in any of the investigated groups to original levels. However the difference betweenits expression on lymphocytes before trypsin treatment and on lymphocytes after 16-hour incubation was higher inthe elderly subjects 16% (p
Key words: ageing, trypsin, treatment, restoration, CD2, CD4, CD8, CD45RA.
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